不同微生物佐剂S180瘤苗的免疫效应和抑瘤作用

Immune Enhancement and Tumor Inhibition of S-180 Vaccine Combined with Different Microorganism Adjuvants

目的 比较肿瘤主动特异性免疫治疗中具有佐剂作用的白色念珠菌、李斯特菌及卡介苗的免疫调节效应和抑瘤作用。方法 选择白色念珠菌、李斯特菌、卡介苗各自联合小鼠S 180肿瘤疫苗 ,皮下免疫接种荷瘤小鼠 ,检测小鼠脾细胞IL 2、IFN 产生水平 ,腹腔MTNF α产生水平 ,观察荷瘤小鼠瘤体重量和存活期。结果 三种微生物佐剂均能协同瘤苗增强免疫小鼠IL 2、IFN 和TNF α的产生能力 (P <0 0 1,0 0 5 ) ,有效抑制足垫部移植瘤的生长 ,抑制率在 6 2 8%～ 75 8%之间 ,显著高于单独注射瘤苗组 (P <0 0 1) ,延长腹腔植瘤小鼠平均存活期 7～ 14天 ,白色念珠菌、李斯特菌协同提高腹腔植瘤小鼠存活率 2 0 %。结论 白色念珠菌和李斯特菌与卡介苗具有同样的佐剂效应 。

Objective To compare the immunoregulation effects and tumor inhibitions of Candida albicans(CA),Listeria monocytogenes(LM),and BCG which were used as adjuvents in the active specific immunotherapy of mice S 180 tumor.Methods S 180 tumor cells were treated with CA or LM and then prepared to vaccines(CA S180 vaccine,LM S180 vaccine).BCG S180 vaccine was a mixture of formalin fixed tumor cell and BCG in proportion.Balb/c mice were inoculated with 1×10 5 S 180 tumor cells in footpads or in abdominal cavities followed by the immunotherapy of the described vaccines.IL 2,IFN  secreted by spleen cells and TNF α secreted by peritoneal M of the immunized mice were determined.The weights of footpad tumors were recorded and the growth inhibitions were calculated.The average survival periods of tumor bearing peritoneally mice were also reckoned.Results The levels of IL 2,IFN ,and TNF α in group CA S180,LM S180,and BCG S180 were much higher than that in control group S 180 and saline ( P <0 01,0 05).The tumor growth in three groups was also inhibited with the range of 62 8%～75 8% inhibition ratios ( P <0 01).The average survival period of mice in three groups enlonged to 7～14 days.CA S180 vaccine and LM S180 vaccine raised respectively the living ratios of tumor bearing mice to 20% (2/8).Conclusions These results indicated that Candida albicans,Listeria monocytogenes play a role in similar immunoenhance effects as BCG and inhibit effectively the growth of experimently transplanted tumor cooperating with S 180 vaccine