神经细胞粘附分子(NCAM)在脑胶质瘤中的表达及其与PCNA的相关性

Relationship between Expression of NCAM and PCNA in Glioma

目的 检测胶质瘤中神经细胞粘附分子 (NCAM)和增殖细胞核抗原 (PCNA)的表达状况 ,以探讨不同病理类型胶质瘤中二者的表达及其相关性。方法 用抗NCAM及抗PCNA单抗对 8例正常脑组织和 5 1例胶质瘤进行免疫组化检测。结果 NCAM在正常脑组织中全部阳性表达 ,Ⅰ～Ⅱ级胶质瘤、Ⅲ级胶质瘤、Ⅳ级胶质瘤中NCAM阳性表达分别为 95 2 % (2 0 /2 1)、5 2 6 % (10 /19)、18 2 % (2 /11) ,阳性细胞含量随肿瘤恶性程度升高反而减少 ;NCAM阳性表达量与PCNA指数呈负相关 (r=- 0 6 7,P<0 0 1)。结论 脑胶质瘤中NCAM表达程度与胶质瘤的临床病理分级和细胞增殖活性呈负相关 。

Objective The expression of NCAM and PCNA in different pathological classification of glioma was examined in order to explore the correlation between NCAM and PCNA.Method NCAM and PCNA were immunostained by SP immunohistochemical method in the sections of 8 normal brain tissues and 51 gilomas.Results All normal brain tissues express NCAM.NCAM expression was positive in 95 2% (20/21),52 6%(10/19),18 2%(2/11) of GradeⅠ～Ⅱ glioma,GradeⅢ glioma,GradeⅣ glioma respectively.The amount of tumor cell positively stained for NCAM decreased with the degree of histologic malignancy.An inverse corrleation was observed between the amount of NCAM expression and PCNA labelling index (r=-0 67, P <0 01).Conclusions The results suggest that the amount of NCAM expression in glioma is an inverse correlation with histologic malignancy and cell proliferative activity of glioma.NCAM low expression might be one of characteristics of malignant glioma.