Cx43蛋白在宫颈癌细胞中的表达及其对细胞通讯功能的影响

Expression of gap junction protein connexin 43 in HeLa cell line and its effect on gap junctional intercellular communication

目的 探讨细胞间隙连接蛋白 Cx43在人子宫颈癌细胞系 He L a中表达 ,及其对细胞间隙连接通讯的影响 .方法 应用流式细胞仪以及 L ucifer Yellow划痕标记荧光传输技术 ,研究维甲酸作用对 He L a细胞 Cx43蛋白表达 ,以及细胞生长状态和间隙连接通讯功能的调节作用 .结果 He L a细胞经维甲酸处理后 ,流式细胞仪分析 ,Cx43蛋白荧光强度明显增强 ,Cx43蛋白表达阳性细胞计数率由 1.9%上升至2 6 .3% ;He L a细胞生长明显受抑 ;细胞间隙连接通讯功能得到恢复 .结论 维甲酸可通过上调 Cx43蛋白在 He L a细胞中的表达 ,使细胞间隙连接通讯功能得到恢复 。

AIM To investage the expression of gap junction protein connexin (Cx) 43 in human cervical carcinoma cell line HeLa and its effect on gap junctional intercellular communication. METHODS Cell culture, flow cytometer (FCM) and Lucifer Yellow scrape loading and dye transfer (SLDT) were employed to detect the expression of Cx43 pro tein in HeLa and the regulatory effect on gap junctional intercellular communication treated by AT retinoic acid (ATRA). RESULTS After treated by ATRA, HeLa cell grew much slower than the untreated ell. FCM found the positive rate of Cx43 in HeLa increased from 1.9% to 26.3%. Gap junction intracellular communication (GJIC) capacity recovered in ATRA treated cells. CONCLUSION The anti tumor effect of ATRA in HeLa may result from the up regulation of Cx43 gene and recovery of Cx43 mediated GJIC.