摘要
目的:研究巨噬细胞迁移抑制因子受体CD74在乳腺癌细胞株MCF-7和MDA-MB-231以及人乳腺癌组织中的表达及其与乳腺癌患者预后的关系。方法:应用荧光定量聚合酶链式反应(fluorescent quantitative polymerase chain reaction,FQ-PCR)和蛋白质印迹(Western blotting)方法分别检测不同转移潜能乳腺癌细胞株MCF-7和MDA-MB-231 CD74的mRNA和蛋白表达量,并比较CD74在两者间的表达差异;应用免疫组织化学检测89例乳腺癌组织中CD74的表达情况,分析CD74与乳腺癌患者总生存率和无瘤生存率的关系。结果:高转移潜能乳腺癌细胞株MDA-MB-231中CD74表达显著高于低转移潜能细胞株MCF-7(P<0.001)。CD74表达水平是乳腺癌总生存率(P=0.001)和无瘤生存率(P<0.001)的独立预测因素。结论:CD74的表达水平与乳腺癌患者预后呈负相关。
Objective：To study the expression level of CD74 receptor of macrophage migration inhibitory factor（MIF） in cell lines and tissues of human breast cancer and its correlation with prognosis. Methods：The expression levels of CD74 mRNA and protein were detected by fluorescent quantitative polymerase chain reaction（FQ-PCR） and Western blotting in breast cancer cell lines MCF-7 and MDA MB-231 which were different in the potential of metastasis;the expression of CD74 in 89 cases of breast cancer was detected by immunohistochemistry. The relationship between CD74 expression and overall survival （OS） and disease-free survival （DFS） of the breast cancer patients was analyzed. Results： The expression level of CD74 in high metastatic potential cell strain MDA-MB-231 was higher than that in the low metastatic potential cell strain MCF-7（P〈0. 001 ）. CD74 ex pression was an independent prognostic factor of OS（P = 0. 001 ）and DFS（P〈0. 001 ）. Conclusions： The expression level of CD74 is an independent prognostic factor in breast cancer.
出处
《中国临床医学》
2012年第5期542-544,共3页
Chinese Journal of Clinical Medicine
