摘要
目的 :探讨热休克蛋白 70 (HSP70 )在细胞创伤后增殖中的作用。方法 :采用细胞机械创伤模型 ,检测创伤后 48h时热处理细胞及非热处理细胞的增殖活性 ,并用免疫细胞化学的方法检测两组细胞生长过程中HSP70的表达。结果 :非热处理组细胞在增殖期表达 HSP70。热处理可诱导 HSP70的表达 ,热处理组创伤后细胞的增殖活性明显高于非热处理组。结论 :HSP70参与细胞增殖过程 ,热处理引起的细胞增殖活性升高可能与 HSP70的功能有关。
sObjectives:To study the role of heat shock protein 70 (HSP70) in cell proliferation after wound.Methods:The mechanical scrape wound model of 3T3 cell was used.Cells were divided into heat pre treated group and non pretreated group.Their proliferation ability at 48 h after wound was measured via MTT assay.The expression of HSP70 was detected by immunocytochemistry.Results:HSP70 strongly expressed when cells were proliferating.After heat pretreated,cells were induced to express HSP70.The A values of heat pretreated cells and non pretrated were 2.15±0.14 and 1.67±0.28 respectively ( P <0.01).Conclusion:HSP70 may play a role in cell proliferation and the enhanced cell proliferation by heat pretreatment may be due to the functions of HSP70.
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2000年第2期122-124,共3页
Journal of Practical Stomatology