丙型肝炎病毒E2基因激发小鼠细胞免疫的实验研究

Study of the cellular immune responses in mice immunized with DNA vector encoding hepatitis C virus E2 protein

目的 :研究丙型肝炎病毒E2基因接种能否诱导机体产生细胞免疫以及乙型肝炎病毒preS基因对其诱导细胞免疫能力的影响。方法 :以BALB/c小鼠的骨髓瘤细胞株 (SP2 /O)为宿主细胞 ,建立表达丙肝病毒E2蛋白的靶细胞 ,将其注射于E2基因或 preS与E2融合蛋白基因免疫的小鼠腹腔 ,记录小鼠接种瘤细胞后的存活期 ,以小鼠存活期的长短作为反映基因免疫小鼠对HCVE2蛋白细胞免疫的有无或强弱的指标。结果 :靶细胞表达了相对分子质量约为 70 0 0 0的E2蛋白 ,E2基因接种小鼠的存活期长于对照组 ,单独E2基因接种组的存活期显著长于对照组 ,也长于 preS与E2融合蛋白基因免疫组。 结论 :E2基因接种能诱导细胞免疫 ,但与 preS融合后 ,其诱导细胞免疫的能力会降低。

Objective:To study the cellular immune responses in mice immunized with the vector expressing E2 protein and find out whether the ability of inducing cellular immunity by E2 gene immunization was affected by HBV preS gene when they were fused. Methods: The target cells were established by transfecting the vector expressing HCV E2 protein into SP 2/O myeloma cells from BALB/c mice, and then injected into the abdominal activity of the mice immunized with HCV E2 gene. The cellular immune response of the E2 gene immunized mice to HCV E2 protein was presented by its survival time after injected target cells. Results: The molecular weight of the HCV E2 protein expressed in the target cell was about 70 000. After injected target cells, the average survival time of the mice in the group immunized with E2 gene alone was longer than that of the group injected with E2 gene fused with HBV preS and was significantly longer than that of the control. Conclusion: Cellular immune responses to HCV E2 protein could be induced by injecting the vector expressing E2 protein into mice, but the ability of E2 gene inducing cellular immunity was reduced when fused with HBV preS. ( J Beijing Med Univ, 2000,32:102 104 )