CD44v6蛋白在宫颈癌中的表达及其与预后因素的关系

The relationship between the expression of CD44v6 protein and prognosis factors in human carcinoma of cervix

目的 :了解CD44v6在宫颈上皮不典型增生、宫颈原位癌、宫颈浸润鳞癌及腺癌中的表达及其与预后因素的关系。方法 :用免疫组化法检测 10例正常宫颈上皮、15例宫颈上皮不典型增生、16例宫颈原位鳞癌、1例宫颈原位腺癌、2 7例宫颈浸润鳞癌、15例宫颈浸润腺癌中CD44v6蛋白的表达 ,实验结果以图像处理与分析系统进行半定量处理后进行统计分析。结果 :(1)着色部位主要为细胞膜 ,细胞浆不着色或着色较浅。细胞核被苏木素复染成淡蓝色。 (2 )CD44v6阳性表达正常宫颈上皮为 1/ 10 ,宫颈不典型增生为 7/ 15 ;宫颈鳞癌中原位癌为 7/ 16 ,浸润癌为2 0 / 2 7;宫颈腺癌中原位癌 1例CD44v6阴性 ;浸润癌 15例 ,CD44v6阳性表达 2 / 15。 (3)宫颈浸润鳞癌淋巴结转移阳性者CD44v6的灰度显著高于淋巴结转移阴性者 [(0 .30 8± 0 .0 2 1)vs (0 .2 73± 0 .0 31) ,P =0 .0 0 6 ]。CD44v6与宫颈鳞癌的临床分期及细胞分化程度无关。结论 :CD44v6在正常宫颈、CIN、宫颈浸润鳞癌中的阳性表达逐渐增加 ;CD44v6与宫颈鳞癌淋巴结转移相关 ;CD44v6阳性可能与宫颈腺癌的不良预后有关 ,其相关性需进一步研究。

Objective: To study the expression of CD44v6 protein in the dysplasia, carcinoma in situ, invasive squamocellular carcinoma and adenocarcinoma of cervix uteri, and the relationship between the expression and the prognosis. Methods: The method of immunohistochemistry was used to study the expression of CD44v6 protein in the cases, including 10 normal cervix, 15 dysplasia, 16 squamocellular carcinoma in situ , 1 adenocarcinoma in situ , 27 invasive squamocellular carcinoma, and 15 invasive adenocarcinoma of cervix uteri, The results were analyzed semi quantitatively with scanning system. And the data were analyzed statistically. Results: (1) The staining reaction of CD44v6 protein was mainly confined to the cellular membrane. (2) Normal epithelial, cervical dysplasia, squamocellular cervical carcinoma in situ and invasive squamocellular carcinoma showed expression of CD44v6 in 1/10, 7/15, 7/16 and 20/27. (3) In the positive cases of pelvic lymphonodes metastasis in invasive squamocellular carcinoma of cervix, the staining reaction of CD44v6 was stronger than that in the negative cases of metastasis in the same disease ( P =0.006). There is no relationship between the expression of CD44v6 and the FIGO stage and carcinoma cellular grade in this study. Conclusion: The degree of staining reaction of CD44v6 increased in the order of normal cervix, Cervical Intraepithelial neoplasia (CIN) and invasive squamocellular carcinoma of cervix; There is really the relationship between the staining reaction of CD44v6 and pelvic lymphonodes metastasis in invasive squamocellular carcinoma; There is perhaps the relationship between the staining reaction of CD44v6 and poor prognosis of cervix adenocarcinoma, but the degree of this relationship should be studied again in more cases. (J Beijing Med Univ, 2000,32:155 157)