血必净对博莱霉素诱导的肺纤维化大鼠病理及血清IL-1β、IL-6、IL-13、TNF-α水平的影响

Effect of Xuebijing on serum cytokine levels and lung pathological changes of rats with bleomycin-induced pulmonary fibrosis

目的研究血必净对博莱霉素诱导的肺纤维化大鼠IL-1β、IL-6、IL-13及TNF—α表达及肺组织病理改变的影响。方法将64只Wistar大鼠随机分成空白组、模型组、血必净组及甲泼尼龙组，每组16只。除空白组外，其余3组以气管内滴注博来霉素（5mg／kg）建立纤维化模型，于造模后第14、28天取材。采用HE及Masson染色法观察肺组织病理变化，酶联免疫吸附（ELISA）法检测血清IL-1β、IL-6、IL-13及TNF-α的含量。结果在第14天时，血必净组肺泡炎程度明显轻于模型组，大鼠肺泡炎积分明显降低（P〈0．05），血清IL-13较模型组明显降低（P〈0．05），TNF—α较模型组明显降低（P〈0．05）；在第28天时，血必净组仅形成轻中度肺间质纤维化，肺纤维化积分较模型组明显降低（P〈0.05）。结论血必净能显著降低血清IL-13及TNF-α表达水平，减轻肺组织肺泡炎及肺纤维化程度，从而抑制或延缓博莱霉素致大鼠肺纤维化的发生、发展。

Objective To investigate the effect of Xuebijing on serum cytokine contents and pathological changes in the lung of rats with bleomycin-induced pulmonary fibrosis. Methods Sixty four Wister rats were randomly divided into four groups （n=16 in each group）： control group, model group, the xuebijing group and methylprednisolone group. The pulmonary fibrosis model was induced by intra-tracheal injection of bleomycin. The animals were sacrificed d14 and d28, the lung tissue was examined by HE staining and Masson staining and the serum contents of IL-1β、IL-6、IL-13、TNF-α were assayed by ELISA. Results Compared with model group, the serum levels of IL-β and TNF- α, and the degrees of alveolitis decreased significantly in Xuebijing group at d14 （P〈0.05）; the pulmonary fibrosis was attenuated at d28 （P〈0.05）. Conclusion Xuebijing can attenuate the alveolitis and pulmonary fibrosis, and decrease serum contents of IL-13 and TNF- α in bleomycin-induced pulmonary fibrosis rats.