鼻咽癌细胞凋亡及凋亡相关基因的表达

Apoptosis and apoptosis related gene expression in nasopharyngeal carcinoma

目的 探讨细胞凋亡在鼻咽癌 (NPC)中的作用。方法 采用TdT酶介导的生物素化dutp缺口末端标记技术 (Tunel)和免疫组化S P法 ,分别检测 2 0例正常鼻咽粘膜组织及 73例NPC组织的细胞凋亡率和p16、bcl 2基因的表达。结果 正常鼻咽粘膜细胞凋亡率显著高于NPC(P <0 .0 1) ,p16、bcl 2在NPC组织中的表达分别为 2 8.8% (2 1/73)、81.2 % (5 9/73) ,在正常粘膜的表达分别为 90 0 % (18/2 0 )、15 0 % (3/2 0 )。NPC中bcl 2表达与癌细胞凋亡率呈负相关 ,而p16则与凋亡率呈正相关 (P <0 .0 1)。NPC的 3年生存率资料表明 ,细胞凋亡率 >2 0、bcl 2表达阴性、p16表达阳性的患者预后较佳。结论 在NPC中 ,癌细胞凋亡明显受到抑制 ,其发生可能与bcl 2及p16基因调控障碍有关。NPC细胞凋亡与患者的预后相关。

Objective To study apoptosis and expression of apoptosis related genes in nasopharyngeal carcinoma(NPC). Methods Paraffin embedded tissue blocks from 73 cases of NPC and 20 cases of chronic nasopharyngitis were used in this study. Using terminal deoxynucleotidyl transferase mediated dUTP biotin nick end labeling (Tunel) and immunohisytochemistry S P method, the apoptotic rate (AR) and expression of p16 and bcl 2 protein were examined.Results Few apoptotic cells were found in NPC, the AR significantly lower than that of chronic nasopharyngitis ( P <0.01). The frequency of expression of p16 and bcl 2 in NPC was 28.8% (21/73) and 81.2% (59/73), respectively, while that in chronic nasopharyngitis was 90% (18/20) and 15%(3/20), respectively. In NPC, expression of p16 was positively correlated, while bcl 2 negatively correlated with the AR ( P <0.01). According to 3 year follow up, the survival rate was higher in NPC patients with AR>2.0 and negative bcl 2 expression, whereas that was higher in patients with positive expression of p16.Conclusion Apoptosis is inhibited in NPC which may be related to p16 and bcl 2 gene dysregulation. Apoptosis correlates with prognosis in NPC patients. Subject