摘要
目的探讨结肠癌中环氧化酶2（COX-2）表达的意义及其与血管r8皮生长因子C（VEGF—C）、微血管密度（MVD）和微淋巴管密度（MLD）的关系。方法采用免疫组化EnVision法检测56例结肠癌中COX-2、VEGF—C、CD34和D2-40的表达。结果COX-2、VEGF—C在结肠癌中的阳性率分别为69．6％（39／56）和66．1％（37／56）；COX-2与组织学分级、浸润深度、淋巴结转移、Dukes分期相关（P〈0．05），与性别、肿瘤大小无关（P〉0．05）；VEGF—C与浸润深度、淋巴结转移、Dukes分期相关（P〈0．05）。与性别、肿瘤大小、组织学分级无关（P〉O．05）：COX-2、VEGF—C表达阳性组的MVD、MLD均明显高于阴性组（P〈0．01）；COX-2与VEGF—C呈正相关（P〈0．01）；MVD与MLD无关（P〉O．05）。结论COX-2可作为结肠癌恶性程度及预后判断的参考指标，其可能通过上调VEGF-C的表达以促进肿瘤血管及淋巴管生成而参与结肠癌的浸润转移。
Objective To investigate the expression of cyclooxygenase-2 （COX-2） and its relationship with angiogene- sis/lymphangiogenesis in colon cancer. Methods Immunohistochemical staining EnVision method was used to detect the expression of COX-2, vascular endothelial growth factor-C（VEGF-C）, CD34 and D2-40 in 56 cases of colon cancer. Results The positive rates of COX-2 and VEGF-C in colon cancer was 69.6% （39/56） and 66.1% （37/56） respectively. The expression of COX-2 was correlated with histological grade, invasive depth, lymphatic metastasis and Dukes＇ stage （P〈0.05）, not with sex and tumor size（P 〉0.05）. The expression of VEGF-C was correlated with invasive depth, lymphatic metastasis and Dukes＇ stage （P〈 0.05）, not with sex, tumor size and histological grade（P 〉0.05）. The microvessel density （MVD） and microlymphatic density （MLD） in cases with positive COX-2 and VEGF-C expression were significantly higher than those without COX-2 and VEGF-C expres- sion （P〈0.01）. The expression of COX-2 was correlated with VEGF-C （P〈0.01）. MVD was not correlated with MLD （P 〉0.05）. Conclusion COX-2 can be used as a reference marker for assessment of differentiation and prognosis of colon cancer, which may be associated with upregulation of VEGF-C and vascularization in the tumors.
出处
《浙江医学》
CAS
2012年第20期1644-1646,共3页
Zhejiang Medical Journal
基金
温州市卫生局科研项目（2011B074）
