鸡骨草总黄酮超声提取工艺及含量测定研究

Optimizatiom of ultrasonic extraction process and determination for total flavonoids in Abrus cantoniensis Hance

目的筛选鸡骨草中总黄酮超声提取的最佳工艺,并建立其含量测定方法。方法以芹菜素-6,8-C-二葡糖苷为指标,采用三乙胺显色-紫外可见分光光度法(TEA-UV-VIS法)测定鸡骨草中总黄酮含量,采用L9(34)正交试验优选鸡骨草总黄酮提取工艺条件。结果芹菜素-6,8-C-二葡糖苷线性范围为0.000 507～0.101 4 mg/mL(r=0.999),平均加样回收率为99.72%(RSD为1.54%)。鸡骨草总黄酮超声提取工艺试验因素影响由大到小依次为超声时间>乙醇体积分数>提取次数>料液比,超声提取时间对提取量的影响有统计学意义,优选的最佳提取工艺为20倍量的50%(体积分数)乙醇超声提取2次,每次45 min,3次试验鸡骨草总黄酮的平均提取量为4.655 mg/g。结论 TEA-UV法具有良好的专属性和稳定性,适用于鸡骨草中总黄酮的含量测定和评价;优选的总黄酮最佳提取工艺方法稳定可行。

Objective To optimize the ultrasonic extraction process and establish a method for the determination of total flavonoids in Abrus cantoniensis Hance.Methods With the content of 6-C-glycopyranosyl-8-C-glycopyranosyl apigenin as index,the total flavonoids were determined by TEA-UV-VIS method,and the extraction technology was optimized by L9（34） orthogonal method.Results The linear range of 6-C-glycopyranosyl-8-C-glycopyranosyl apigenin was 0.000 507-0.101 4 mg/mL（r=0.999）,and the average recovery was 99.72%（RSD=1.54%）.The influencing factors of ultrasonic extraction arran-ged in order were as follows： ultrasonic extracting timeethanol concentrationextracting timessolid-liquid ratio,and ultrasonic extracting time was the significant influence factors.Optimal technology was： ultrasonic extraction twice with 20 times of 50% ethanol by 45 minutes each time.The average amount of total flavonoids was 4.655 mg/g.Conclusion TEA-UV method had good specificity and stability and can be used to determine and evaluate the total flavonoids in Abrus cantoniensis Hance.The optimized process was stable and available.