HPLC法测定人血浆中表柔比星的浓度

Determination of Epirubicin in Human Plasma by HPLC

目的:建立人血浆中表柔比星浓度的测定方法。方法:采用HPLC法测定,色谱柱为Waters XTerra C18(250 mm×4.6 mm,5μm),流动相为乙腈-水(30∶70,pH 2.5),柔红霉素为内标,荧光检测波长为λex=465 nm,λem=550 nm,样品在碱性条件下,用乙酸乙酯液液萃取、浓集后进样。结果:表柔比星在0.02～40μg·ml-1浓度范围内线性关系良好(r=0.999 5,n=12),日内RSD<3.20%,日间RSD<4.71%,方法回收率95.28%～104.62%。结论:该方法简便可靠、专属性好、精密度高,可用于人血浆中表柔比星浓度的测定。

Objective： To establish a method for the determination of epirubicin in human plasma by HPLC.Method： The HPLC method was carried out on a Waters symmtry C18（250 mm×4.6 mm,5 μm）column using acetonitrile-water（30∶ 70,pH 2.5）as the mobile phase,and the detection wavelengths was λex=465 nm and λem=550 nm.The alkalized sample was extracted with ethylacetate1.After evaporation of the organic layer,the residue was dissolved by the mobile phase and determined.Result： The calibration curve of epirubicin was linear over the range of 0.02-40 μg·ml-1（r=0.999 5,n=12）.The inter-day precision was less than 3.20% and the intra-day precision was less than 4.71%.The method recovery was 95.28%-104.62%.Conclusion： The method is simple,accurate and reproducible,and it can be used in the determination of epirubicin in human plasma.