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NKG2D、MICA和IL-8在早期自然流产组织中的表达及意义

Expression and implication of NKG2D, MICA, and IL-8 in villi and decidua of spontaneous abortus at first trimester
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摘要 目的探讨NKG2D、MICA和IL-8在早期自然流产(ESA)患者蜕膜绒毛中的表达及其意义。方法选取20例ESA患者作为流产组,20例正常早孕人工流产妇女作为对照组,RT-PCR和Western Blotting法检测两组蜕膜绒毛组织中NKG2D、MICA和IL-8mRNA及蛋白的表达,免疫组织化学法检测蜕膜绒毛组织中MICA蛋白的表达,分析NKG2D、MICA与IL-8之间的相关性。结果流产组蜕膜绒毛组织中NKG2D、MICA和IL-8mRNA表达水平低于对照组;流产组蜕膜绒毛组织中NKG2D、MICA和IL-8蛋白表达低于对照组,差异均有统计学意义。MICA存在于绒毛组织合体滋养层细胞、细胞滋养层细胞及蜕膜组织基质细胞中。NKG2D、MICA与IL-8mRNA呈正相关(r=0.71、0.74,P〈0.05);NKG2D、MICA与IL-8蛋白呈正相关(r=0.76、0.77,P〈0.05)。结论ESA患者蜕膜绒毛组织中NKG2D、MICA和IL-8mRNA和蛋白表达水平低,这可能是导致滋养细胞侵袭性降低并发生ESA的重要原因。 Objective To investigate he expression and implication of natural-killer group 2 member D (NKG2D), MHC class I chaln-related molecule A (MICA), and Interleukin-8 (IL-8) in villi and decidua of first trimester spontaneous abortus. Methods Expression of NKG2D, MICA, and IL-8 mRNA and protein in villi and decidua of first trimester spontaneous abortus ( abortion group, n = 20) and normal first trimester pregnant women ( control group, n = 20) was detected by semi-quantitative RT-PCR and Western blot. MICA protein was detected by immunohistochemistry. Results Expression of NKG2D, MICA, and IL-8 mRNA was significantly lower in villi and decidua in the abortion group compared with that in the control group (P 〈 0.05 ). Expression of NKG2D, MICA, and IL-8 protein in villi and decidua in the abortion group was significantly lower than that in the control group ( P 〈 0.05 ). Expression of NKG2D and MICA in villi and decidua at first trimester has significantly positive correlation with the expression of IL-8. Immunohistochemistry showed that MICA existed in the villous syncytiotrophoblast cells and decidual stromal cells. Conclusion Lower expression of NKG2D and IL-8 in villi and decidua of early spontaneous abortus may be associated with the decrease in the ability of early embryo invasion. It may lead to spontaneous abortion during early pregnancy.
出处 《中国生育健康杂志》 2012年第6期417-421,共5页 Chinese Journal of Reproductive Health
关键词 早期自然流产 自然杀伤细胞2D受体 MHC—I类链相关分子A 白介素-8 Early spontaneous abortion NKG2D MICA IL-8
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