NKG2D、MICA和IL-8在早期自然流产组织中的表达及意义

Expression and implication of NKG2D, MICA, and IL-8 in villi and decidua of spontaneous abortus at first trimester

目的探讨NKG2D、MICA和IL-8在早期自然流产（ESA）患者蜕膜绒毛中的表达及其意义。方法选取20例ESA患者作为流产组，20例正常早孕人工流产妇女作为对照组，RT-PCR和Western Blotting法检测两组蜕膜绒毛组织中NKG2D、MICA和IL-8mRNA及蛋白的表达，免疫组织化学法检测蜕膜绒毛组织中MICA蛋白的表达，分析NKG2D、MICA与IL-8之间的相关性。结果流产组蜕膜绒毛组织中NKG2D、MICA和IL-8mRNA表达水平低于对照组；流产组蜕膜绒毛组织中NKG2D、MICA和IL-8蛋白表达低于对照组，差异均有统计学意义。MICA存在于绒毛组织合体滋养层细胞、细胞滋养层细胞及蜕膜组织基质细胞中。NKG2D、MICA与IL-8mRNA呈正相关（r=0．71、0．74，P〈0．05）；NKG2D、MICA与IL-8蛋白呈正相关（r=0．76、0．77，P〈0．05）。结论ESA患者蜕膜绒毛组织中NKG2D、MICA和IL-8mRNA和蛋白表达水平低，这可能是导致滋养细胞侵袭性降低并发生ESA的重要原因。

Objective To investigate he expression and implication of natural-killer group 2 member D （NKG2D）, MHC class I chaln-related molecule A （MICA）, and Interleukin-8 （IL-8） in villi and decidua of first trimester spontaneous abortus. Methods Expression of NKG2D, MICA, and IL-8 mRNA and protein in villi and decidua of first trimester spontaneous abortus （ abortion group, n = 20） and normal first trimester pregnant women （ control group, n = 20） was detected by semi-quantitative RT-PCR and Western blot. MICA protein was detected by immunohistochemistry. Results Expression of NKG2D, MICA, and IL-8 mRNA was significantly lower in villi and decidua in the abortion group compared with that in the control group （P 〈 0.05 ）. Expression of NKG2D, MICA, and IL-8 protein in villi and decidua in the abortion group was significantly lower than that in the control group （ P 〈 0.05 ）. Expression of NKG2D and MICA in villi and decidua at first trimester has significantly positive correlation with the expression of IL-8. Immunohistochemistry showed that MICA existed in the villous syncytiotrophoblast cells and decidual stromal cells. Conclusion Lower expression of NKG2D and IL-8 in villi and decidua of early spontaneous abortus may be associated with the decrease in the ability of early embryo invasion. It may lead to spontaneous abortion during early pregnancy.