摘要
[ Objective] To prepare specific monoclonal antibodies against salbutamol （SAL） and thus to provide the basis for the immunological detection. [Method] Balb/c mice were immunized with SAL-BSA. Hybridoma strains （SAL rnAb） secreting monoclonal antibody against SAL were established through cell fusion. Ascites of SAL mAb were prepared in vivo and the immunological characteristics were observed. [Resalt] Two hybridoma strains, SAL-2D9 and SAL-1C4, were established, and the indirect ELISA titers of the cell supematant and ascites were 1：1 × 10^4 and 1 ： 1 × 10^6, respectively. Their isotype was IgGl. The median inhibitory concentration （IC5o） of the SAL mAb against SAL was 1.14 ng/ml. It had cross-reactivity of 100% against SAL and 26.09% against clenobuterol hydrochloride, and no cross-reaction was found between the SAL mAb and other SAL analogues. [ Conclusion] The high-titer, sensitive and specific anti-SAL monoclonal antibodies have been produced, which makes it possible to develop immunoassay for detection of SAL residues.
[ Objective] To prepare specific monoclonal antibodies against salbutamol （SAL） and thus to provide the basis for the immunological detection. [Method] Balb/c mice were immunized with SAL-BSA. Hybridoma strains （SAL rnAb） secreting monoclonal antibody against SAL were established through cell fusion. Ascites of SAL mAb were prepared in vivo and the immunological characteristics were observed. [Resalt] Two hybridoma strains, SAL-2D9 and SAL-1C4, were established, and the indirect ELISA titers of the cell supematant and ascites were 1：1 × 10^4 and 1 ： 1 × 10^6, respectively. Their isotype was IgGl. The median inhibitory concentration （IC5o） of the SAL mAb against SAL was 1.14 ng/ml. It had cross-reactivity of 100% against SAL and 26.09% against clenobuterol hydrochloride, and no cross-reaction was found between the SAL mAb and other SAL analogues. [ Conclusion] The high-titer, sensitive and specific anti-SAL monoclonal antibodies have been produced, which makes it possible to develop immunoassay for detection of SAL residues.
基金
funded by the Key Technology R&D Program of Hebei Province (09227134D)
Key Technology R&D Program of Shijiazhuang City (10150142A)
