摘要
To study the effects of 17beta-estradiol (E2 ) and progesterone (P4) levels on superovulatory response and recipients conception rate, the E2 and P4 levels of embryo donor (Borderdale) and recipient ewes (Inner Mongolian sheep) were determined during multiple ovulation and em- bryo transfer (MOET). The results showed that the E2 level increased from the day of initiation by CIDR (Controlled internal drug release devices) treatment and decreased to 3.18± 1.32 pg/ml on the day of superovulatory estrus. On the day of embryo collection, the ewes having higher levels of estrogenic peak showed better superovulatory response than the other groups having comparatively lower levels. On the day of initiation of super- ovulatory treatment, the ewes having lower levels of P4 showed better superovulatory response than those having higher levels of P4. The E2 level of the early abortion group increased from Day 0 to Day 45, while that of two pregnant recipient groups came down from Day 0 to Day 30. The ser- um P4 level of the early abortion group was significantly lower than that of the pregnant recipient groups on Day 45 ( P 〈 0.05), but no significant difference was found between the two pregnant groups after Day 30. Therefore, the levels of E2 and P, on definite time affect superovulatory re- sponse of donors and conception rate of recipients during MOET.
To study the effects of 17beta-estradiol (E2 ) and progesterone (P4) levels on superovulatory response and recipients conception rate, the E2 and P4 levels of embryo donor (Borderdale) and recipient ewes (Inner Mongolian sheep) were determined during multiple ovulation and em- bryo transfer (MOET). The results showed that the E2 level increased from the day of initiation by CIDR (Controlled internal drug release devices) treatment and decreased to 3.18± 1.32 pg/ml on the day of superovulatory estrus. On the day of embryo collection, the ewes having higher levels of estrogenic peak showed better superovulatory response than the other groups having comparatively lower levels. On the day of initiation of super- ovulatory treatment, the ewes having lower levels of P4 showed better superovulatory response than those having higher levels of P4. The E2 level of the early abortion group increased from Day 0 to Day 45, while that of two pregnant recipient groups came down from Day 0 to Day 30. The ser- um P4 level of the early abortion group was significantly lower than that of the pregnant recipient groups on Day 45 ( P 〈 0.05), but no significant difference was found between the two pregnant groups after Day 30. Therefore, the levels of E2 and P, on definite time affect superovulatory re- sponse of donors and conception rate of recipients during MOET.
基金
funded by Agricultural Research Institute"948"Program of China(2000-2005)