摘要
[Objective] To investigate the optimal determination conditions of melamine in animal blood products by high performance liquid chromatography （HPLC）. [ Method] The blood samples were extracted with ultrasonic in 1% trichloroacetic acid （TDA） and acetonitrile. After purifying by solide phase extraction （SPE）, the samples were analyzed by H PLC. r Result I The optimal conditions of HPLC were as follows： the chromatographic column was Zorbax SB-CS; the mobile phase was ion-pairs buffer-acetonitrile （95/5, V/V） ; the flow rate was 1.0 ml/min; the column temperature was 25 ℃ and the UV detection wavelength was 235 nm. The determined melamine concentration range was 0.001 -0.050 mg/ml; the linear correlation coefficient was 0.999 4; the concentration limit of melamine was 0.1 mg/kg; the average recovery rate of the melamine were 97.60% - 100.65%, and the relative standard deviation （RSD） was 1.23% -3.04%.[ Conclusion] The HPLC is simple, accurate and repeatable for determination of the melamine in animal blood products.
[Objective] To investigate the optimal determination conditions of melamine in animal blood products by high performance liquid chromatography （HPLC）. [ Method] The blood samples were extracted with ultrasonic in 1% trichloroacetic acid （TDA） and acetonitrile. After purifying by solide phase extraction （SPE）, the samples were analyzed by H PLC. r Result I The optimal conditions of HPLC were as follows： the chromatographic column was Zorbax SB-CS; the mobile phase was ion-pairs buffer-acetonitrile （95/5, V/V） ; the flow rate was 1.0 ml/min; the column temperature was 25 ℃ and the UV detection wavelength was 235 nm. The determined melamine concentration range was 0.001 -0.050 mg/ml; the linear correlation coefficient was 0.999 4; the concentration limit of melamine was 0.1 mg/kg; the average recovery rate of the melamine were 97.60% - 100.65%, and the relative standard deviation （RSD） was 1.23% -3.04%.[ Conclusion] The HPLC is simple, accurate and repeatable for determination of the melamine in animal blood products.
基金
supported by the Shanghai Key Development Project of Agriculture Science and Technology (2009 No.6-3)
