摘要
[ Objective] To understand the toxicity and sterilization effects of the tetrakis hydroxmethyl phosphonium sulfate （THPS） as a novel quatemary phosphonium biocide. [ Method] Under hydrostatic conditions, the acute toxicity and bactericidal property of THPS on the Fiexibacter coiumnaris （ F. columnaris） were observed in Japanese eel. The semi-lethal concentration （LCso）, safe concentration （Sc）, minimum inhibitory concentration （MIC）, minimum bactericidal concentration （MBC） and the sterilization rate of THPS were calculated, respectively. [ Result ] The LCso of THPS at 24, 48 and 96 h were 11.9, 9.9 and 9.1 mg/L, respectively. The Sc of THPS at 24 h was 2.1 mg/L; the MIC and MBC of THPS to the F. columnaris were 0.36 and 0.72 mg/L; and the sterilization rate was 100% at a concentration of 0.7 mg/L at 12 h. The sterilization rate of THPS had no great change with pH values varying from 5.5 to 9.5. [ Condusion] The THPS is a novel, safe and effective biocide for prevention and treatment of bacterial diseases of Japanese eel.
[ Objective] To understand the toxicity and sterilization effects of the tetrakis hydroxmethyl phosphonium sulfate （THPS） as a novel quatemary phosphonium biocide. [ Method] Under hydrostatic conditions, the acute toxicity and bactericidal property of THPS on the Fiexibacter coiumnaris （ F. columnaris） were observed in Japanese eel. The semi-lethal concentration （LCso）, safe concentration （Sc）, minimum inhibitory concentration （MIC）, minimum bactericidal concentration （MBC） and the sterilization rate of THPS were calculated, respectively. [ Result ] The LCso of THPS at 24, 48 and 96 h were 11.9, 9.9 and 9.1 mg/L, respectively. The Sc of THPS at 24 h was 2.1 mg/L; the MIC and MBC of THPS to the F. columnaris were 0.36 and 0.72 mg/L; and the sterilization rate was 100% at a concentration of 0.7 mg/L at 12 h. The sterilization rate of THPS had no great change with pH values varying from 5.5 to 9.5. [ Condusion] The THPS is a novel, safe and effective biocide for prevention and treatment of bacterial diseases of Japanese eel.
基金
supported by the Public Welfare Specialized Research Fund,Ministry of Agriculture ( 200803013)
the Director Fund of Pearl River Fisheries Research Institute( 2007-G-6)
