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Isolation and Identification of Pandora neoaphidis and Its Pathogenicity against Turnip Aphid(Lipaphis erysimi)

萝卜蚜新蚜虫疠霉的分离鉴定及其致病性测定(英文)
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摘要 [Objective] The study was to isolate and identify the pathogen of Pandora neoaphidis and to determine its pathogenicity against turnip aphid (Lipaphis erysimi).[Method] Based on the morphological characteristics,the species of pathogenic fungus was identified.Spore shower method was used to carry out bioassay on the pathogen against turnip aphid.[Result] Primary conidia were ovoid,bitunicate and uninucleate,(24.7±1.4)μm×(10.7±0.9)μm,L/D=2.3±0.2.Secondary conidia had the similar shape with the primary ones,(18.6±2.1)μm×(13.3±1.3)μm,L/D=1.4±0.2.Hyphal body was like mycelium with the diameter of (10.6±0.8)μm.Conidiophores had palmate branch with the diameter of (10.0±0.9)μm.Pseudocystidia was not branched,which had rough base with the diameter of (19.2±1.7)μm,and gradually became more angular towards the apex with the diameter of (8.0±0.9)μm at tips.Rhizoid was like monohyphal shape with the diameter of (21.0±3.0)μm at base,the terminal apex had regular discoid holdfast.No resting spores were observed.The lethal dose of the pathogen against turnip aphid was 18.2/mm2.[Conclusion] The entomopathogenic fungus against turnip aphid was identified to be Pandora neoaphidis,and the pathogen was confirmed to have strong pathogenicity against turnip aphid. [Objective] The study was to isolate and identify the pathogen of Pandora neoaphidis and to determine its pathogenicity against turnip aphid (Lipaphis erysimi).[Method] Based on the morphological characteristics,the species of pathogenic fungus was identified.Spore shower method was used to carry out bioassay on the pathogen against turnip aphid.[Result] Primary conidia were ovoid,bitunicate and uninucleate,(24.7±1.4)μm×(10.7±0.9)μm,L/D=2.3±0.2.Secondary conidia had the similar shape with the primary ones,(18.6±2.1)μm×(13.3±1.3)μm,L/D=1.4±0.2.Hyphal body was like mycelium with the diameter of (10.6±0.8)μm.Conidiophores had palmate branch with the diameter of (10.0±0.9)μm.Pseudocystidia was not branched,which had rough base with the diameter of (19.2±1.7)μm,and gradually became more angular towards the apex with the diameter of (8.0±0.9)μm at tips.Rhizoid was like monohyphal shape with the diameter of (21.0±3.0)μm at base,the terminal apex had regular discoid holdfast.No resting spores were observed.The lethal dose of the pathogen against turnip aphid was 18.2/mm2.[Conclusion] The entomopathogenic fungus against turnip aphid was identified to be Pandora neoaphidis,and the pathogen was confirmed to have strong pathogenicity against turnip aphid.
作者 贾春生
出处 《Plant Diseases and Pests》 CAS 2010年第6期43-45,共3页 植物病虫害研究(英文版)
基金 Supported by Key Projects in Shaoguang Institute (200402)~~
关键词 Pandora neoaphidis PATHOGENICITY Turnip aphid Entomopathogenic fungus ENTOMOPHTHORALES Pandora neoaphidis Pathogenicity Turnip aphid Entomopathogenic fungus Entomophthorales
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