乙肝表面抗原、e抗原定量检测与Pre-S1Ag的相关性分析

Hepatitis B Surface Antigen,E Antigen Quantitative Detection Pre-S1Ag Correlation Analysis

目的:探讨HBsAg、HBeAg定量检测与Pre-S1Ag的相关性和临床意义。方法:540例乙肝患者血清,经处理,分别进行酶联免疫吸附试验(ELISA)法检测Pre-S1Ag和时间分辨荧光定量PCR法检测HBsAg、HBeAg。对HBsAg、HBeAg(-)组别、HBeAg(+)组别以及Pre-S1Ag进行χ2检验。结果:540份血清标本定量检测显示:(1)E抗原HBeAg(+)组的HBeAg浓度为(49.77±30.78)PEIU/ml,Pre-S1Ag(+)率为81.12%;E抗原HBeAg(-)组的HBeAg浓度为(0.14±0.33)PEIU/ml,Pre-S1Ag(+)率为21.60%;乙肝表面抗原HBsAg(+)组的HBsAg浓度为(139.78±89.10)PEIU/ml,Pre-S1Ag(+)率为35.00%。HBeAg(+)组和Pre-S1Ag无统计学意义,P>0.05;HBeAg(-)组、HBsAg(+)组和Pre-S1Ag具有统计学差异,P<0.05。(2)HBeAg(+)HBsAg(+)HBcAb(+),HBsAg浓度为(212.30±46.90)PEIU/ml,Pre-S1Ag(+)率为81.12%;HBeAb(+)HBsAg(+)HBcAb(+),HBsAg浓度为(139.78±89.10)PEIU/ml,Pre-S1Ag(+)率为27.95%。结论:通过检测Pre-S1Ag可以了解乙肝患者体内是否存在病毒复制情况。

Objective：To explore the relevance and clinical significance of HBsAg,HBeAg quantitative detection of the Pre-S1Ag.Methods：540 cases of patients with hepatitis B were treated enzyme-linked immunosorbent assay（ELISA） assay the the Pre-S1Ag and time-resolved fluorescence quantitative PCR assay HBsAg,HBeAg.Theχ2 test for HBsAg,HBeAg（-） group,HBeAg（＋） group and the Pre-S1Ag.Results：The quantitative detection of 540 serum samples：（1） E antigens of HBeAg（＋） HBeAg concentration group（49.77 ± 30.78） PEIU / ml,Pre-S1Ag（＋） was 81.12%;the the E antigen HBeAg（-） group HBeAg concentration（0.14±0.33） PEIU / mlPre-S1Ag（＋） rate was 21.60%;hepatitis B surface antigen HBsAg（＋） group HBsAg concentration（139.78 ± 89.10） PEIU / ml,the Pre-S1Ag（＋） was 35.00%.HBeAg（＋） group and the Pre-S1Ag non-statistically significant,P 0.05;HBeAg（-） group,HBsAg（＋） group and the Pre-S1Ag a statistically significant difference,P0.05.（2） HBeAg（＋） HBsAg（＋） HBcAb（＋） HBsAg concentration（212.30 ± 46.90） the PEIU / ml,the Pre-S1Ag（＋） was 81.12%;HBeAb（＋） of HBsAg（＋） HBcAb（＋）,HBsAgconcentration（139.78 ± 89.10） PEIU / ml,Pre-S1Ag（＋） was 27.95%.Conclusion：By detection Pre-S1Ag can understand the presence of the virus of hepatitis B patients in vivo replication.