乌司他丁对重度失血性休克大鼠脑缺血再灌注相关因子的影响

Effects of Ulinastatin on Cerebral Ischemia/Reperfusion-related Factors after Severe Hemorrhagic Shock in Rats

目的:研究乌司他丁对重度失血性休克大鼠复苏后血清和脑组织中丙二醛(MDA)、超氧化物歧化酶(SOD)和肿瘤坏死因子o(TNF-α)含量的影响。方法:将42只大鼠随机分为假手术组(A),单纯缺血再灌注组(B)和乌司他丁治疗组(C),通过颈动脉放血建立失血性休克模型,B、C组根据休克时间分为30、60、120 min三个亚组,各组大鼠在复苏后测定血清和脑组织中MDA、SOD和TNF-α的含量。结果:大鼠休克30 min复苏血清MDA和TNF-α含量较A组升高,但C组低于B组,血清SOD和脑组织中MDA、SOD和TNF-α含量未出现明显变化;大鼠休克60 min复苏血清MDA、TNF-α含量进一步升高,脑组织TNF-α含量开始升高,但C组低于B组;大鼠休克120 min复苏,血清和脑组织MDA、TNF-α含量较A组明显升高,C组低于B组,血清和脑组织SOD活力明显下降,但C组高于B组。结论:在重度失血性休克情况下进行复苏,大鼠将出现全脑缺血再灌注损伤,应用乌司他丁可在一定程度上减轻该损伤。

Objective：To study the effects of Ulinastatin on content of serum and cerebral MDA, SOD and TNF- α after severe hemorrhagic shock and resuscitation in rats. Methods： 42 rats were randomly divided into three groups as A （sham operation group）,B（ischemia-reperfusion group） and C （Ulinastatin treated group）.The hemorrhagic shock mouse model was reproduced by withdrawal of blood from carotid artery. Gruop B and C were divided into three subsets according shock time course：30 min,60 min and 120 min subset. The content of serum and cerebral MDA,SOD and TNF-α in each group were measured after resuscitation.Results： Among the 30 min shock rats with resuscitation, serum MDA and TNF-α of group B and C was higher than group A, and group C was lower than group B, there wasn＇t significant change of serum SOD and cerebral MDA, SOD and TNF-α. Among the 60 min shock rats with resuscitation, serum MDA and TNF-α become increasingly higher. Cerebral TNF-α begin to increase, and group C was lower than group B. Among the 120 min shock rats with resuscitation, MDA and TNF-α in serum of group B and C was significantly higher than group A, and group C was lower than group B,SOD in both serum and brain decreases obviously, but group B was higher than group C. Conclusion：Rats resuscitated from severe hemorrhagic shock will appear the global cerebral ischemia-reperfusion injury, the application of Ulinastatin can reduce the damage to some extent.