丹参提取物对CCl_4诱导建鲤肝损伤血清和肝脏生化指标的影响

Effects of Biochemical Indexes of Salvia Extract against CCl_4-induced Liver Injury in Serum and Liver of Jian Carp

为了研究丹参提取物对建鲤肝损伤生化指标的影响,选取体质健康、无伤的建鲤120条,随机分成6组,每组20条。5个处理组,1个对照组。每日饲喂2次,连续饲喂8周。I组(CCl4)、II组(0.5%丹参提取物)、III组(1%丹参提取物),按0.05 mL/10g体重体内注射30%CCl4;IV组(0.5%丹参提取物)、V组(1%丹参提取物)体内不注射CCl4,只注射同体积植物油;VI为空白对照组,也注射同体积植物油。注射72h后采集建鲤的血清及肝组织进行化验。结果表明:与I组(CCl4)相比,III组(1%丹参提取物)血清中丙氨酸转氨酶(GPT)、天冬氨酸转氨酶(GOT)、乳酸脱氢酶(LDH)的活性显著降低(P<0.05);总蛋白(TP)、白蛋白(ALB)的活性有所增加(P>0.05);超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)含量显著提高,丙二醛(MDA)含量显著降低(P<0.05);总抗氧化能力(T-AOC)和过氧化氢酶(CAT)含量显著提高(P<0.05)。而IV,V组的上述指标则无明显变化,这说明1%丹参提取物对于抗CCl4诱导的肝损伤有较好的效果。

In order to study the protective effects of Salvia extract anti fish liver injury, 120 Jian carps which without injury and healthy were selected as objects for studying, they were randomly di- vided into six groups, 20 fish each, five treatment groups, one control group. They were fed two times a day, continued feeding for 8 weeks. Ⅰ （CC14） , Ⅱ （0.5% Salvia extract）, Ⅲ （1% Salvia extract） group were injected （ip） with 30% （v/v） of CC14 in arachis oil at a volume of 0.05ml/10g of body weight; IV （0.5% Salvia extract） and V （ 1% Salvia extract） control group had no CCl4 injection, according to the weight injected the same volume of arachis oil; VI was the blank control group, according to the weight injected the same volume of arachis oil. 72 h after injection, the serum and liver tissue of all groups were taken for the test. The results showed that： compared with the Ⅰ （CC14 ） group, Ⅲ（ 1% Salvia extract） group could significantly reduce the activity of GPT, GOT and LDH in serum, significant difference （ P 〈 0.05 ） ; increased the activity of TP, ALB, significant difference （ P 〉 0.05 ） ; could significantly improve the content of SOD, GSH-Px in liver tis- sues, reduced the content of MDA, significant difference （ P 〈 0.05 ） ; could significantly improve the content of T-AOC and CAT, significant difference （ P 〈 0.05 ）. But the IV and V group had no significant change ; this showed that 1% Sa＇Ivia extract had a good effect in against CCl4-induced liver injury.