LED不同光质对虎雪兰组培苗生理生化特性影响的研究

Effect of LED Different Light Qualities on Physiological and Biochemical Caracteristics of Cymbidium tracyanum var. huanghua×Cymbidium mastersii Tissue Culture Seedlings

本试验在同等光强条件下研究LED不同光质对虎雪兰组培苗生理生化特性的影响,希望能够为植物生产类LED光源的参数设置和系统研发提供数据支持和理论依据。结果表明:(1)RBW(红∶蓝∶白=6∶1∶1)处理下虎雪兰组培苗整株干重最大,为0.098 2 g;对照组W1(白色荧光灯)根部干重最低,为0.082 3 g。(2)RBG(红∶蓝∶绿=4∶2∶1)处理下叶绿素a,叶绿素b和类胡萝卜素含量极显著高于其他处理(P<0.01),分别为0.438 0,0.159 0,0.091 0 g,利于虎雪兰中上述3种色素的合成。(3)B(单色蓝光)处理下叶片淀粉粒积累最少,为0.062 2 g,而其游离氨基酸和可溶性蛋白含量却是最高的,分别为140.997 1,5.001 4μg/g;而R(单色红光)处理下游离氨基酸和可溶性蛋白含量均最低,分别为99.455 7,2.726 9μg/g。综合比较其中RBG处理下虎雪兰组培苗长势最好,而R和B处理下长势相对较差,光质对虎雪兰幼苗生长代谢中的影响比较明显,LED光照系统可以替代荧光灯成为虎雪兰幼苗组织培养的理想光源。

In order to study growth metabolism influence of Cymbidium tracyanum var. huanghua × C. mastersii tissue culture seedlings under the same light intensity, to provide data support and theoretical basis of light-emitting diode （LED） light parameters settings and system research and development, the experiment was conducted. The results showed ： （ 1 ） with treatment RBW （ red ： blue ： white = 6： 1：1 ）, dry weight of the whole plant of seedlings cultivation was the biggest, with 0. 098 2 g; Dry weight of the roots control group W1 （fluorescent lamp or fluorescent） was minimum, accounting for 0. 082 3 g. （2） RBG （ red： blue： green = 4： 2：1 ） with chlorophyll a, b and chlorophyll content of ca- rotenoids significant was higher than other treatments, being 0. 438 0, 0. 159 0. 0. 091 0 g respec tively, and beneficial to the three pigments synthesis of seedlings. （3） B （ monochrome blue-ray） accumulation of leaf starch grains was the least, with 0. 062 2 g, and the free amino acids and soluble protein content was the highest, respectively 140. 997 1, 5. 001 4 μg/g; And R （monochrome red light） with free amino acids and soluble protein content were the lowest, respectively 99. 455 7, 2. 726 9 μg/g. Comprehensive comparison among them, RBG treatment was the best for seedlings growing, and R and B growing was poor relatively. C. tracyanum var. huanghua× C. mastersii tissue culture seedlings growth in the metabolism was influenced markedly by light quality, LED illumination system could replace fluorescent become a ideal light source in C. tracyanum var. huanghua × C. mastersii tissue culture seedlings tissue culture.