回转条件下微生物产酶表征

Enzymatic Characteristics of Microbial Fermentation Under Clinostat Rotation

选定3株高产淀粉酶、蛋白酶、纤维素酶的细菌(枯草芽孢杆菌)、放线菌(链霉菌)和丝状真菌(里氏木霉)作为模式菌株,研究了在回转条件下单独发酵产酶及混合培养产酶过程及能力。结果表明,枯草芽孢杆菌、链霉菌和里氏木霉在pH值6.0～7.9之间都可生长。在回转条件下发酵30d,枯草芽孢杆菌维持一定产淀粉酶和蛋白酶能力;链霉菌能够产少量的蛋白酶和淀粉酶;里氏木霉除了具有一定的产纤维素酶能力外,也能产少量的蛋白酶和淀粉酶。将3株菌进行分步混合培养,发酵过程中均能保持较高的淀粉酶活力和蛋白酶活力,在20d和30d的发酵液中检测不到纤维素酶活力。

Bacillus subtilis, Streptomycete and Trichoderma reesei were selected for production of amylase, proteinase （keratinase） and eellulase under clinostat rotation. It was found that the three strains could grow on plates with in the pH range from 6.0 to 7.9. Bacillus subtilis and Streptomycete maintained low level of amyl- ase and proteinase, respectively. Trichoderma reesei could produce all three kinds of enzymes. When mixing the three microbes together as a eo-euhure,both amylase and proteinase were produced at a high level during 20 d or 30 d under clinostat rotation,while no eellulase was detected. The above results provided possible strategies for the degradation of organic wastes from the life system in the future CELSS.