半夏泻心汤及其拆方抗幽门螺杆菌作用的研究

Antibacterial effects of Banxia Xiexin decoction and its separated recipes on Helicobacter pylori

目的:探讨半夏泻心汤及其拆方抗Hp的作用。方法:运用琼脂扩散法、液体稀释法定性和定量检测半夏泻心汤及其拆方对Hp的抗菌作用。结果:在琼脂扩散法中,半夏泻心汤组对Hp抗菌作用优于辛温组和甘温组,差异有统计学意义(P<0.05);半夏泻心汤组略优于苦寒组,差异无统计学意义(P>0.05)。在液体稀释法中,半夏泻心汤对Hp的最低抑菌浓度(MIC)与最低杀菌浓度(MBC)均为15.63mg/mL;辛温组和苦寒组对Hp的最低抑菌浓度(MIC)与最低杀菌浓度(MBC)均分别为62.50 mg/mL、31.25mg/mL。均在实验检测的浓度范围内(1.95 mg/mL～250.00 mg/mL)。结论:半夏泻心汤有较明显的抗菌作用,其中起优势作用的药物以苦寒药为主。

Objective ： To investigate the antibacterial effects of Banxia Xiexin decoction and its separated recipes on Helicobacter pylori （Hp）. Methods： Agar diffusion and broth dilution methods were used to qualitatively and quantitatively evaluate the antibacterial effects of Banxia Xiexin decoction and its separated recipes on Hp. Results： The agar diffusion method showed that Banxia Xiexin decoction exhibited significantly tvetter antibacterial effect than pungent and warm - natured recipe and sweet and warm - natured recipe （P 〈 O. 05 ） ; Banxia Xiexin decoction was slightly better than bitter and cold recipe （ P 〉 O. 05 ）. The broth dilution method showed that Banxia Xiexin decoction had minimal inhibitory concentration （MIC）/minimum bactericidal concentration （MBC） of 15.63 mg/ml for Hp, while the pungent and warmnatured recipe and bitter and cold recipe had MIC/MBC of 62.50 mg/ml and 31.25 mg/ml, respectively, all in the test concentration range （1.95 -250. 00 mg/ml） Conclusion： Banxia Xiexin decoction has good antibacterial effect on Hp, with bitter and coht recipe as the dominant effective component.