牛不同组织中稳定性同位素氢、氧、硫组成探讨

STABLE HYDROGEN,OXYGEN AND SULFUR ISOTOPES COMPOSITION IN DIFFERENT TISSUES OF CATTLE

为研究牛不同组织中氢、氧、硫稳定同位素的组成变化情况以及品种对其的影响,利用同位素比率质谱仪测定了不同部位脱脂牛肉、牛尾毛、血液、肝脏中的δ2H值和δ34S值及牛肉抽提水中的δ2H,δ18O值。结果显示,品种因素不影响组织中的硫同位素组成,但对氢同位素组成的影响尚不确定;肌肉不同部位、血液、肝脏、牛尾毛之间的δ2H值、δ34S值均有极显著差异,但各组织间的δ34S、δ2H值相关关系不明显;肉品水中δ2H值与δ18O值存在较强的相关关系。这些结果说明稳定性同位素氢、硫在牛不同组织中的分馏效应是不一致的,在进行牛肉溯源研究时,应根据研究目的和研究对象选择合适的组织。

In order to research on stable hydrogen,oxygen,sulfur isotopes composition in different tissues of cattle,as well as the breed,δ2H and δ34S values of different defatted muscle,cattle tail hair,blood,liver,also δ2H and δ18O values of water from muscle were determined by isotope ratio mass spectrometry.The stable sulfur isotope composition was not affected by cattle variety,meanwhile the hydrogen was uncertain;the δ2H and δ34S values between different defatted muscle,blood,liver,cattle hair were significantly different,at the same time the δ34S and δ2H values between each tissue were not significantly correlated;the δ2H values were strongly correlated with the δ18O values of muscle water.The above results indicated that stable sulfur and hydrogen isotopes fractionation in the various tissues were discrepant,thus the proper tissue should be selected according to the purpose and object in the beef traceability.