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人结直肠癌细胞Annexin A2基因敲除细胞系的建立 被引量：1

The generation of Annexin A2-/-Caco2 cell line
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摘要 为研究ANXA2与结直肠癌发展的关系以及其在肿瘤发展过程中作用的构效关系,构建了ANXA2基因敲除质粒重组子,然后导入人结直肠癌细胞系Caco2,经过一系列筛选过程,最终建立了基因敲除ANXA2的Caco2细胞系(ANXA2-/-Caco2).经过PCR和Western Blotting检测,确认该细胞系无任何野生ANXA2表达. To study the roles played by ANXA2 during the development of colorectal carcinoma, and the relationship between the structure and function of ANXA2, ANXA2 knockout recombinant was constructed and transfected into Caco2 cell line. After a series of selecting step, ANXA2 --/--Caco2 cell line was successfully generated. By the designated PCR and Western blot analysis, it was confirmed that the wild type ANXA2 expression is knockout completely in this cell line.
作者 邢睿欢 牟茂森 高晓杰 聂红娟 杨敏敏 徐竹 熊艳 吴琴丰 陈亦雯 宋铭 侯颖春
机构地区 陕西师范大学生命科学学院
出处 《陕西师范大学学报（自然科学版）》 CAS CSCD 北大核心 2012年第6期61-66,共6页 Journal of Shaanxi Normal University：Natural Science Edition
基金 陕西省自然科学基金资助项目(SJ08-ZT09)
关键词 AnnexinA2 基因敲除 Caco2细胞 Annexin A2 gene knockout Caco2 cell
分类号 Q291 [生物学—细胞生物学]
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参考文献27

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同被引文献11

  • 1吴相钰,陈守良,葛明德等.陈阅增普通生物学.第3版.北京:高等教育出版社,2009.
  • 2Alberts B.,Johnson A.,Lewis J.,et al.Molecular Biology of the Cell,5th edition.USA.2008.
  • 3陈晔光,张传茂,陈俭.分子细胞生物学.第2版.北京:清华大学出版社,2011.
  • 4Lewin B..Genes ⅤⅢ.Pearson Education,Inc.USA.2004.
  • 5Irie N.,Kuratani S..Comparative transcriptome analysis reveals vertebrate phylotypic period during organogenesis.Nat Commun,2011,2:248.
  • 6Wyrwicz L.S.,yon Grotthuss M.,Pas J.,Rychlewski L..How unique is the rice transcriptome?.Science,2004,303(5655):168.
  • 7Tseng C.K.,Cheng S.C..Both catalytic steps of nuclear pre-mRNA splicing are reversible.Science,2008,320(5884):1782-1784.
  • 8Carrington J.C.,Ambros V..Role of microRNAs in plant and animal development.Science,2003,301(5631):336-338.
  • 9Yingchun Hou,Lijuan Yang,Maosen Mou,et al.Annexin A2 regulates the levels of plasminogen,S100A10 and fascin in L5178Y cells.Cancer Invest,2008,26:809-815.
  • 10Yingehun Hou,Yang Hou,Siyu He,etal.The Novel insights into spatiotemporal cell biology and its schematic frame,Triple W.J Cell Physiol,2012,227(5):1787-1790.

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陕西师范大学学报（自然科学版）
2012年 第6期

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