丰头皮海绵化学成分研究

STUDIES ON THE CHEMICAL CONSTITUTIONS OF THE SPONGE SUBERITES TYLOBTUSA LEVI

采用体外细胞毒活性追踪方法 ,应用低压柱层析 ,中压柱层析 ,高效液相等色谱技术从采自西沙永兴岛的丰头皮海绵 ( Suberitestylobtusa Levi)脂溶性部分中分得了 18个化合物。运用多种光谱技术鉴定了其中7个化合物 ,分别为对羟基苯甲酸 ,对羟基苯乙酰胺 ,3-吲哚甲酸 ,3-吲哚甲醛 ,6-溴 - 3-吲哚甲酸 ,胸腺嘧啶脱氧核苷 ,邻苯二甲酸二丁酯。其中对羟基苯乙酰胺文献报道具有一定的抗肿瘤活性 ,体外对小鼠淋巴性白血病细胞 ( P388)的 ED50 值为 0 .5μg·m L-1,体内对淋巴细胞性白血病小鼠 T/C值为 140 % [1]。

Guided by in vitro cytotoxic activity, multi-chromatography methods are employed. Eighteen compounds have been isolated form the lipid friction of the sponge suberites [CM(40]tylobtusa Levi. The seven of them:phydroxybenzoic acid, phydroxyphenylacetamide, indolyl3carboxylic acid,indolyl3carbaldehyde,6bromoindole3carboxylic acid,thymidine,dibutylphthalate were identified by analysis of IR,Mass,1HNMR, 13 CNMR spectral data.Among them:phydroxyphenylacetamide was reported with antitumor activity,against P 388 in vitro ED 50 =0.5μg·mL -1 ,against P 388 in vivo T/C=140%.