湛江地区淋球菌流行株质粒谱型的研究

Study on the plasmid profiles of epidemic Neisseria gonorrhoeae strains in Zhanjiang

为探讨淋球菌耐药谱与质粒谱之间的相互关系 ,对 1998年～ 1999年广东省湛江地区分离鉴定出的 98株淋球菌 ,以碱裂解法进行质粒的抽提 ,并分析质粒谱型的分布情况。结果显示 98株淋球菌流行株 ,检出质粒的有 90株 ,占91 84% ;质粒谱型 12种 ,以 7 4kb +4 2kb ,3 9 5kb +7 4kb +4 2kb为主 ,占3 8 76%。从而揭示湛江地区淋球菌流行株质粒的分布现状 ,为该地区淋病的分子流行病学研究打下了基础 ,但质粒谱与耐药性之间的关系尚有待进一步探讨。

In order to probe the plasmid profiles(PP) and their distributions of N gonorrhoeae, the plasmids of 98 gonococcal strains obtained from 1998 through 1999 in Zhanjiang(Guangdong) were extracted by alkaline lysis technique Then analysis of the distributions of plasmids profiles was performed The results showed that 90 strains of N gonorrhoeae harboured plasmids, the detected percentage accounting for 91 84% There were 12 types of PP The PP types of 7 4kb+4 2kb and 39 5kb+7 4kb+4 2kb were prominent(38 76%) The study indicated the plasmid distribution of N gonorrhoeae in Zhanjiang and made a basis for the molecular epidemiological study of gonorrhea in this area The relationship between PP and the resistance to antimicrobials has to be investigated in future