氧化亚铁硫杆菌的固定化及其对Fe^(2+)的氧化研究

Study on Immobilization of Thiobacillus Ferrooxidans and Oxidation of Fe^(2+)

以沸石为填料、气液逆流形式的固定化细胞装置填料塔,操作条件控制为空气量0.5 m3/h、循环液体流量1.0 L/h,在培养基的初始pH=2.0、Fe2+初始浓度为8 g/L左右的条件下,氧化亚铁硫杆菌固定化细胞只需10 h就达95%以上Fe2+的氧化率,其Fe2+平均氧化速率高达0.88 g/(L.h),固定化细胞Fe2+平均氧化速率是游离细胞的11倍。固定化细胞经长期低pH值驯化后,仍能保持较高的Fe2+氧化活性,在初始pH=1.6的条件下,只需14h Fe2+氧化率就达95.18%,Fe2+平均氧化速率达0.63 g/(L.h);是驯化前Fe2+平均氧化速率的1.58倍。

With zeolite as filler, the gas-liquid countercurrent immobilized cells device as the filling tower, O. 5 m3/h as operating air flow, 1.0 L/h as liquid flow, initial pH =2. 0 and initial Fe2＋ concentration for about 8 g/L, above 95% of ferrous ions are oxidized by immobilized cells in 10 hours. The average oxidation rate is pH adaptati up to 0. 88 g/ （L · h）, which is eleven times as high as that of free cells. After long-term low on, the immobilized cells can keep high oxidation activity of ferrous ions. Under the condition of initial pH = 1.6, 95. 18% of ferrous ions can be oxidized within 14 hours, and the average oxidation rate is up to 0. 63 g/ （ L · h）, which is 1.58 times as high as that of before adaptation.