赤土茯苓苷对小鼠海马CA_1区神经元的缺血再灌注损伤的保护作用

Protective effect of Smiglabran on cerebral ischemia reperfusion damage of neural cell in CA_1 region of hippocumpus in mice

目的 :研究赤土茯苓苷 (Smiglabran,Smi)对小鼠海马 CA1 区神经元的缺血再灌注损伤的保护作用。方法 :采用结扎双侧颈总动脉造成小鼠脑缺血再灌注损伤 ,分别观察了不同再灌注时间 (1、3、5 d)小鼠的存活率及海马 CA1 区神经元的普通病理改变 ,同时观察了赤土茯苓苷对上述改变的影响。结果 :Smi10 0、2 0 0 mg/ kg能明显提高脑缺血后再灌流 3d、5 d小鼠的存活率 ,脑缺血再灌注的早期 ,海马 CA1 区神经元数目及形态即发生改变 ,再灌注 5 d时光镜下绝大部分细胞脱失 ,而用药组 (Smi10 0、2 0 0 mg/ kg)小鼠海马 CA1 区神经元在相应时间点上形态数目变化较轻 ,5 d时仍有超过半数以上 (6 8.1%、74.1% )细胞存活 ,细胞密度分别为 (95 .4± 8.0 )个 / 2 mm和 (10 3.8± 3.2 )个 / 2 mm,明显高于缺血对照组 (5 1.8± 7.8)个 / 2 mm,P <0 .0 0 1。结论 :Sm i对小鼠短暂脑缺血再灌注造成的海马 CA1

Objective: To study the protective effect of Smi on ischemia reperfusion damage of neural cell in CA\-1 region of hippocumpus in mice. Methods: Acute brain ischemia reperfusion injury was produced by reversible occlusion of the bilateral carotid arteries, to observe the effect of Smi on the survival rate of mice and the general pathological changes of neural cell in CA\-1 region reperfused for different days. Resulus: Smi 100\,200 mg·kg -1 increased the survival rate of mice reperfused for 3 and 5 days. At early stage of reperfusion, the number and form of neusal cells in CA\-1 region were changed, and on reperfusion for 5 days later, the most number of eclls were disappeared. The changes of Smi 100\,200 mg/kg groups were less obvious than the I R group. On the fifth day, more than half of nural cells (68.1%,74.1%) still survived in CA\-1 region. The cell density was 95.4±8.0,103.8±3.2个/2 mm, which was higher than I R group obviously (P< 0.001) . Conclusion: Smi has protective effect against ischemia reperfusion damage of neural cell in CA\-1 region in mice.