油酸致ARDS过程脂质过氧化和一氧化氮的动态变化

Dynamic changes of myocardiac lipid peroxidation and NO during oleic acid-induced ARDS

目的 :观察急性呼吸窘迫综合征 (ARDS)合并左心功能降低时血浆和心肌组织脂质过氧化和一氧化氮(NO)的动态变化 ,以了解它们在左心功能降低中的作用。方法 :用油酸缓慢 (iv:耳缘静脉 )注射诱发兔肺损伤所致ARDS模型并分为 30 min、6 0 min、90 min和 12 0 min4组。结果 :(1)油酸各时间组血浆和心肌组织的 GSH- Px和MDA含量与对照组相比有显著差异 (P <0 .0 5～ 0 .0 1) ,而且二者呈负相关 (r =- 0 .98,P <0 .0 1) ;(2 )油酸 30min和 6 0 min组血浆 NO含量高于对照组 (P <0 .0 1) ,在油酸 90 min和 12 0 min组比油酸 6 0 min组又有显著减少 (P <0 .0 1) ;(3)心肌组织 NO含量在油酸 30 min时比对照组明显增加 (P <0 .0 1) ,而在油酸 6 0 min以后各组与油酸 30 min组相比又有所减少 (P <0 .0 1) ;(4 )电镜下见到心肌细胞结构有明显改变。 结论 :油酸致 ARDS过程左心功能降低与脂质过氧化和

Objective: Observing the dynamic changes of myocardiac lipid peroxidation and nitric oxide (NO) during acute respiratory distress sydrome (ARDS) with decreasing of cardiac function and their role in decreasing of cardiac function were assessed. Methods: The experimental animals were divided into 4 groups of OA30 min,OA60 min, OA90 min and OA120 min. Result: (1) the content of GSH Px and MDA in all OA groups showed a notable discrepancy compared with control group (P<0.05～0.01) and there was a negative correlation between GSH Px and MDA; (2) the content of NO in plasma at 60 min and 90 min was higher than the control group (P<0.05), but it was lower at 90 min and 120 min than 60 min(P<0.05); (3) the content of NO in heart at OA 30 min was higher than the control group (P<0.05), but it was lower at OA 60min,90min and 120 min than OA 30 min (P<0.05); (4) alterations of myocardiac ultrastructure were seen under the electron microscope. Conclusion: there is secondary myocardiac damage and decreased function during oleic acid induced ARDS in the rabbits. The changes are associated with myocardiac lipid peroxidation and nitric oxide.