乳腺癌间质新生血管周细胞的形态学特点及其意义

Ultrastructural and immunohistochemical characteristics of pericytes during neovascularization in breast carcinoma

目的 研究乳腺癌间质新生血管周细胞形态学特点、特异性标记及其与内皮细胞的关系 ,并定量分析周细胞与血管密度的关系。方法 应用超微结构、免疫组织化学LSAB法及形态定量 ,对 89例乳腺癌及 4例新鲜肉芽组织新生血管的周细胞进行系统形态学观察。结果 内皮细胞第八因子相关抗原 (FⅧRAg)表达阳性 ,胞质内含有特征性的Ⅷ因子小体 (Weibel paladebody)。周细胞α 平滑肌肌动蛋白 (α SMA)表达阳性 ,胞质内含有丰富的肌丝 ,与内皮细胞间可见连接。癌间质内新生血管的周细胞与血管密度的比值 ,低血管密度区 (1.4± 0 3)明显高于高血管密度区 (4 .3± 0 .9) ,P <0 .0 0 0 5。结论 α SMA免疫组织化学染色结合部位和超微结构可作为研究周细胞的有用指标 。

Objective To study the morphology of pericyte, the relationship between pericytes and neovascularization of breast carcinoma. Methods Ultrastructural, immunohistochemical and quantitative morphology analysis techniques were used to study 89 cases of human breast carcinoma. 4 human granulation tissues cases were used as control. Results The positive cells for FⅧRAg were endothelial cells situated inside the vascular wall with abundant cytoplasm containing caveolae and Weibel palade bodies. The positive cells for α SMA were pericytes with cytoplasm containing abundant myofilament, and connections with endothelial cells. The pericyte endothelial junctions can be found. Quantitative morphological analysis showed that the number of pericytes in the low microvascular density areas are much higher than that in the high microvascular density areas ( P <0.000 5). Conclusion Ultrastructural observation and immunohistochemical staining of α SMA are useful in identifying pericytes. The results of this study imply that pericytes may possibly inhibit angiogenesis.