血必净对急性胰腺炎大鼠血清中TNF-α、IL-1β和IL-6浓度的影响

Treatment with Xuebijing injection decreases serum concentrations of TNF-α,IL-1 β and IL-6 in rats with acute pancreatitis

目的:检测血必净治疗前、后急性胰腺炎(acute pancreatitis,AP)大鼠血清细胞肿瘤坏死因子(tumor necrosis factor α,TNF-α)、白细胞介素(interleukin,IL)-1β和IL-6浓度变化、胰腺组织损伤的关系,以探讨血必净缓解AP大鼠炎症反应的可能机制.方法:♂SD大鼠90只分为对照组(A组,n=30),AP组(B组,n=30)和血必净治疗组(C组,n=30);腹腔注射L-精氨酸制造AP大鼠模型,观察模型成功制作后6、12及24h各组大鼠血清细胞因子TNF-α、IL-1β和IL-6浓度变化,采用酶联免疫吸附剂测定(ELISA)法测定血清细胞因子TNF-α、IL-1β和IL-6的浓度.胰腺组织病理变化并对胰腺组织损伤进行评分.结果:与A组比较,B组大鼠6、12及24h血清TNF-α、IL-1β和IL-6浓度升高,各时间点差异有统计学意义(TNF-α:6h:t=17.72,P=0.00;12h:t=11.19,P=0.00;24h:t=13.70,P=0.00;IL-1β:6h:t=9.61,P=0.00;12h:t=8.69,P=0.00;24h:t=8.08,P=0.00.IL-6:6h:t=29.18,P=0.00;12h:t=18.59,P=0.00;24h:t=17.14,P=0.00).B组大鼠6h、12h及24h胰腺病理评分明显升高,各时间点差异有统计学意义(6h:t=17.23,P=0.00;12h:t=18.72,P=0.00;24h:t=16.08,P=0.00).与B组比较,血必净注射液可明显降低C组大鼠12、24h血清TNF-α、IL-1β和IL-6浓度,12、24h组间差异有统计学意义(TNF-α:12h:t=7.56,P=0.001;24h:t=7.30,P=0.00.IL-1β:12h:t=8.76,P=0.00;24h:t=8.07,P=0.00.IL-6:12h:t=7.82,P=0.00;24h:t=6.72,P=0.00);并且明显降低C组24h大鼠胰腺病理评分分值,24h点组间差异有统计学意义(24h:t=2.79,P=0.015).结论:血必净在早期能够降低AP大鼠血清细胞因子TNF-α、IL-1β和IL-6的浓度.通过抑制炎症反应来改善胰腺组织病理损伤,这可能是血必净治疗AP的作用机制之一.

AIM： To investigate the relationship between serum concentrations of TNF-α, IL-1β, and IL-6 and severity of pancreatic tissue injury and to investigate the possible mechanism by which Xuebijing relieves inflammatory responses in rats with acute pancreatitis （AP）.METHODS： Ninety male SD rats were randomly and equally divided into three groups： control group, AP model group, and treatment group. The rats in the AP model group and treatment group received two intraperitoneal injections of L-arginine （3.2 g/kg body weight） at an interval of 1 h to induce AP. At 6, 12 and 24 h after APinduction, serum levels of TNF-α, IL-1β and IL-6 were determined by ELISA, and pathological changes in pancreatic tissue were scored.RESULTS： Serum concentration of TNF-α, IL-1β and IL-6 at 6, 12 and 24 h in the AP model group were significantly higher than those in the con- trol group （TNF-α： t = 17.72, 11.19, 13.70, all P = 0.00; IL-1β： t = 9.61, 8.69, 8.08, all P = 0.00; IL-6： t = 29.18, 18.59, 17.14, all P = 0.00）. Pathological scores of pancreatic tissue at 6, 12 and 24 h in the AP model group were significantly higher than those in the control group （t =17.23, 18.72, 16.08, all P = 0.00）. Compared to the AP model group, treatment with Xuebijing injection significantly decreased serum levels of TNF-α, IL-1β, and IL-6 at 12 and 24 h （TNF-α： t = 7.56, 7.30, P = 0.001, 0.00; IL-1β： t = 8.76, 8.07; both P = 0.00; IL-6： t = 7.82, 6.72, both P = 0.00） and pathological scores of pancreatic tissue at 24 h （t = 2.79, P = 0.015）.CONCLUSION： Xuebijing injection is effective in attenuating pancreatic tissue injury in rats with AP via mechanisms possibly associated with de- creasing serum levels of TNF-α, IL-1β and IL-6.