甘草酸苷对乙型肝炎疫苗无应答者PBMC细胞因子mRNA表达的影响

Effect of glycyrrhizin on mRNA expression of cytokines in PBMCs from non-responders to hepatitis B vaccine

目的:观察甘草酸苷(Glycyrrhizin,GL)对乙型肝炎疫苗无应答者外周血单个核细胞(peripheral blood mononuclear cell,PBMC)细胞因子mRNA表达的影响,探讨GL增强机体免疫功能的作用机制.方法:分离乙型肝炎疫苗无应答者的PBMC,加入HBsAg和/或GL的条件下体外培养,CCK-8法测定PBMC的增殖能力,real-time PCR测定刺激后IFN-γ、IL-4、IL-10 mRNA的表达水平,并与应答组比较.结果:GL可刺激提高乙型肝炎疫苗无应答者PBMC的增殖能力,使之与应答者无显著性差异(F=3.104,P=0.138).HBsAg+GL+无应答组PBMC的IFN-γ、IL-10mRNA表达水平均高于HBsAg+无应答组,差异有统计学意义(q分别为34.93和26.18,均P<0.05),趋向于应答者分泌水平,GL+无应答组IFN-γ与IL-10的表达均高于HBsAg+无应答组(q分别为10.18和9.19,均P<0.05),但低于HBsAg+GL+无应答组(q分别为24.75和16.98,均P<0.05);而IL-4mRNA变化在无应答者3组间无统计学意义(P=0.304).结论:GL可以调节乙型肝炎疫苗无应答者的免疫状态,创造有利于保护性抗体产生的环境.

AIM： To investigate the effect of glycyrrhizin （GL） on the mRNA expression of cytokines in peripheral blood mononuclear cells （PBMCs） from nonresponders to hepatitis B vaccine and to explore the mechanism by which GL regulates immune function.METHODS： PBMCs were isolated from non- responders to hepatitis B vaccine and incubated with GL and （or） HBsAg. The abilities of GL and （or） HBsAg to stimulate the proliferation of PBMCs were evaluated by CCK-8 assay. The mRNA expression levels of IFN-y, IL-4, andIL-10 were determined by real-time PCR.RESULTS： GL enhanced the proliferation of PBMCs from non-responders to hepatitis B vaccine and made it comparable to that of PBMCs from responders （F = 3.104, P = 0.138）. The mRNA expression levels of IFN-y and IL-10 in PBMCs from non-responders that were incu- bated with HBsAg and GL were significantly higher than those incubated with HBsAg alone （q = 34.93, 26.18, both P 〈 0.05）, and tend to reach the levels in PBMCs from responders. The mRNA expression levels of IFN-y and IL-10 in PBMCs from non-responders that were incu- bated with GL were higher than those incubated with HBsAg （q = 10.18, 9.19, both P 〈 0.05）, but lower than those incubated with HBsAg and GL （q = 24.75, 16.98, both P 〈 0.05）. The mRNA ex- pression levels of IL-4 had no obvious difference among the three groups （P= 0.304）.CONCLUSION： GL can regulate the immune status of non-responders to hepatitis B vaccine and create an environment conducive to the generation of protective antibodies.