水稻Dof基因家族的组织表达谱及胁迫诱导表达特征分析

Transcriptional Profiling Analysis of OsDof Gene Family in Various Rice Tissues and Their Expression Characteristics under Different Stresses

基因的表达与调控是一个复杂的分子网络,受许多转录因子的时空表达调节。Dof蛋白是一类植物特有的转录因子,在多种植物特有生物学过程中起着关键的调控作用。水稻全基因组中发现含30个Dof基因,但对其绝大部分基因的功能尚缺少了解。本研究对30个水稻Dof基因及启动子区进行生物信息学分析,发现OsDof基因上游1.5kb启动子区含有大量的光反应、组织器官表达、植物激素反应及逆境反应等重要的顺式作用元件。进一步通过荧光定量RT-PCR技术分析,结果表明了大部分OsDof基因呈多器官较低丰度表达,少部分为器官优势或高丰度表达。另外,与未胁迫处理的水稻幼苗相比较,28个OsDof基因的表达受光、ABA、NaCl或PEG等胁迫处理的调节,其中27个OsDof基因可以对2种或2种以上的胁迫条件产生响应,表明这些成员可能在生理水平上参与不同胁迫相关信号途径之间的交互作用。

Regulation of gene expression is a complex molecular network controlled by temporal and spatial expression of many transcription factors（TF）.Dof proteins are a family of plant-specific transcription factors,which play important roles in many biological processes restricted to the plants.30 of Dof genes were found in the rice（Oryza sativa） genome,but few of them have been functionally characterized.In this study,We performed a bioinformatics analysis of 30 OsDof genes and their 5＇ flanking promoter sequences.Four kinds of cis-elements were detected to be overrepresented in the 1.5 kb upstream regions of OsDofs,including light-responsive elements,tissue-expressed elements,hormone-responsive elements and stress-responsive elements.Furthermore,qRT-PCR analysis revealed that most OsDof genes were low and multiple-organ expression,and few of them were highexrpessed or tiusse-preferential expression.In addition,Compared to the control without stress treatment,28 OsDof genes were regulated by the stress treatments of light,ABA,NaCl or PEG.Out of them,27 OsDof genes were responsive to two or more types of the above stress treatments,which suggested that these genes should involve in cross-talking among pathways of different singnal transduction.