过量表达拟南芥NPR1基因提高小麦纹枯病的抗性

Over-expression of AtNPR1 Enhances Resistance to Wheat Sharp Eyespot in Transgenic Wheat

拟南芥NPR1基因是植物重要的抗病调控因子,转基因过表达该基因可以赋予植物广谱抗性。为明确该基因在小麦抗纹枯病基因工程中的应用潜力,本研究构建了由玉米ubiquitin启动子驱动的拟南芥NPR1表达载体,采用基因枪介导法导入到小麦品种扬麦12号中,获得20株转基因植株。对14个外源基因纯合株系的半定量RT-PCR和测序分析结果显示,外源拟南芥NPR1基因已经导入转基因小麦并有不同水平的表达,部分转基因株系拟南芥NPR1基因发生重排;纹枯病抗性鉴定结果表明,转基因株系中拟南芥NPR1基因的正确表达可以减轻纹枯病菌引起的病症发展,提高转基因小麦的纹枯病抗性。

The Arabidopsis NPR1 gene is the important antiviral regulatory factor in plant,transgenic overexpression of the gene can be imparted to the plant broad resistance.In order to evaluate the potential application of AtNPR1 gene in the genetic engineering for the resistance to wheat sharp eyespot,the expression vector of AtNPR1 driven by maize ubiquitin promoter was constructed and introduced into wheat Yangmai12 via particle bombardment in this study.Twenty transgenic plants were obtained by L-PPT screening and PCR analysis.The result of semi-quantitative RT-PCR and DNA sequencing of 14 transgenic homozygous lines showed that the alien AtNPR1 genes were integrated into wheat genome and expressed at different levels.Rearrangement of AtNPR1 gene was observed in some transgenic lines.The assessment of resistance to Rhizoctonia cerealis indicated that the correct expression of AtNPR1 in transgenic lines could reduce the severity of wheat sharp eyespot.