抑制COMT基因表达对转基因烟草木质素合成的影响

The Effects of Depressing Expression of COMT on Lignin Synthesis of Transgenic Tobacco

将绿竹COMT(咖啡酸-O-甲基转移酶)基因(BoCOMT1)反向连接到植物表达载体PBI121上,通过农杆菌介导法导入烟草。PCR检测表明,BoCOMT1基因已反向整合到烟草的基因组中。转基因茎杆在剥皮后呈现淡红色,而对照为白色。与对照相比,转基因烟草的木质素含量都有或多或少的下降,其中下降程度最大的达到了28.7%。部分转基因植株出现生长矮小现象。

The COMT（caffeic acid-O-methyltransferase） gene from green bamboo（Bambusa oldhamii）,BoCOMT1,which was aligned reversely in the plant expression vector PBI121,was transformed into tobacco through Agrobacterium-mediated technique.PCR assay showed that BoCOMT1 gene has been integrated into the genome of tobacco.The peeled stems of transgenic lines appeared in pink,while the control appeared in white.Compared to control,lignin contents of transgenic lines were declined at different degree,of which the biggest decline reached 28.7%.Some transgenic plants were growing slowly and appearing dwarfing.