高效液相色谱法测定白菜中甲基对硫磷农药残留量

Determination of Residual Amount of Parathion-meth Pesticides in Cabbage by High Performance Liquid Chromatography

本文采用高效液相色谱法对白菜中甲基对硫磷分离和检测,对样品的提取、净化以及检测的色谱条件进行筛选和优化。以shim-pack VP-ODS为分离柱;流动相为V甲醇∶V水=85∶15,紫外检测波长为265nm.该方法所测得的甲基对硫磷在0～2.0mg/L内具有良好的线性,加标回收率在99.8%～110.0%。该方法稳定可靠,回收率高,适合各种蔬菜中甲基对硫磷残留量的分析。

In this paper,residues of organophosphorous pesticides parathion-methy was analyzed and separated by high performance liquid chromatography.The analysis conditions were optimized for the extraction,purification and separation of samples.Separation was performed in shim-pack VP-ODS column with an eluent consisting of methanol-water（volume ratio 85：15） mixture.The eluate was detected by UV detector at 265 nm.The experimental data had a good characteristic of linearity in the range of 0-2.0 mg/L,The recovery ration ranged from 99.8% to 110.0%.The method proved to be stable and could be used to analyse parathion-methy in different vegetables.