微波固化治疗肝癌抗肿瘤再生长的实验研究

Experimental study of suppress effect of microwave tissue coagulation on the reinoculated H_(22) cell

目的：探讨微波固化治疗肝癌对肿瘤再生长的抑制作用。方法：制作H22鼠肝癌皮下移植模型，对肿瘤分别进行微波固化（A组）、手术切除（B组）及不治疗（C组）3种处理，然后对3组动物分别再次皮下接种不同数量的H22癌细胞。观察再接种癌灶的发癌情况。结果：再接种癌细胞后两个月内发癌率依再接种癌细胞数量而不同。接种107细胞的A、E、C组发癌率分别为25．0％、26．3％和77．8％，C组显著增高（P＜0．01），A、B组相近。接种106细胞，3组发癌率分别为6．3％、20．0％、55．0％，C组与A、B组有显著差异（P＜0．01，P＜0．05），同时A组发癌率比B组已有较大幅度降低。接种细胞数为105时，A组无一例发癌，B、C组发癌率分别为18．7％、50．0％，B组与C组无显著性差异，A组显著低于C组（P＜0．01），A组与B组的差异较接种106细胞时更大（P＜0．086）。结论：微波固化和手术切除治疗实验性肝癌后，均在一定程度上抑制再接种癌细胞生长，其中微波固化的作用强于手术切除。

Objective: To determine the suppress effect of microwave coagulation therapy on the reinoculated H22 liver cancer. Methods: The tumor model was established by hypodermic inoculation of H22 hepatic carcinoma cells and treated with microwave coagulation (A group), surgical resection(B group ) or non-treatment (C group). The three groups were reinoculated subcutaneously with different amount of H22 carcinoma cells respectively. The cancer incidence of the reinoculated tumors was observed. Results: The cancer incidence was diminished with the decrease number of reinoculated cancer cells. When the number of the reinoculated cells was 107, the 2-month cancer incidences of the reinoculated tumor in group A, B and C were 25.0%,26. 3% and 77. 8%, which were almost equal in group A and B but significanly higher in group C(P < 0. 01). When the number of the reinoculated cells was 106, the tumor incidences of the three groups were 6. 3%, 20. 0%, 55. 0% respectively. The incidence in C group was significantly higher in group C than that in A and B (P < 0. 01 and P <0. 05). The difference between A and B was more remarkable. When the number of the reinoculated cells was 105 , the incidences of the three groups were 0. 0%, 18. 7%, 50. 0% respectively . There was no significant difference in cancer incidence between B and C but the incidence in A was significantly lower than that in C (P < 0. 01).The difference between A and B was more remarkable in 105 than in 106. Conclusion: Both microwave tissue coagulation (MTC) and surgical resection exhibit the ability of resisting repeated challenge with viable tumor cells but in varying degrees. The ability of MTC was stronger than that of surgical resection.