hTERT基因反义核酸对K562细胞端粒酶活性的影响

Effect of hTERT antisense oligodeoxynucleotide on telomerase activity in K562 cells

目的：探讨人类端粒酶逆转录酶（humantelomerasereversetranscriptase,hTERT）基因的反义寡核苷酸（antisenseoligodeoxynucleotide，ASODN）对K562细胞端粒酶活性的影响。方法：采用多聚酶链反应-酶联免疫测定（PCR－ELISA）方法检测人工合成的反义脱氧寡核苷酸处理K562细胞前后端粒酶活性的改变，以逆转录PCR（RT－PCR）分析hTERT基因mRNA表达水平。结果：hTERT基因的ASODN作用于K562细胞后，hTERTmRNA表达水平显著下调，其端粒酶活性明显受到抑制。结论：hTERT基因的ASODN可以在翻译水平显著抑制K562细胞的端粒酶活性，hTERT基因与端粒酶的活性密切相关。

Objective: To evaluate the effect of human telomerase reverse transcriptase (hTERT) gene antisense oligodeoxynucleotide (ASODN) on telomerase activity in K562 cells. Methods: Telomerase activity was determined by poymerase chain reaction enzyme-linked immunoassay (PCR-ELISA ) in K562 cells untreated or treated with ASODN, and hTERT mRNA expression was analyzed by reverse transcriptase polymerase chain reaction (RT-PCR). Results: The hTERT mRNA level was decreased, while telomerase activity was significantly inhibited when the K562 cells were treated with ASODN for 48h. Conclusion: It is suggested that hTERT ASODN might specifically inhibit telomerase activity of K562 cells at translation level, and it is further proved that hTERT gene has significant correlation with telomerase activity.