可注射温敏淫羊藿苷壳聚糖水凝胶促进家兔桡骨骨折的愈合

Promotive effect of injectable thermo-sensitive icariin chitosan hydrogel on radial fracture healing in rabbits

背景:淫羊藿可显著促进体外培养成骨细胞的增殖、分化和成熟,刺激骨形成。目的:观察可注射温敏淫羊藿苷壳聚糖水凝胶修复家兔桡骨骨折愈合的效果。方法:麻醉下将36只兔左右桡骨截断,随机分为实验组、对照组和空白组,前两组分别在骨折端周围注入可注射温敏携淫羊藿苷壳聚糖水凝胶、壳聚糖水凝胶,空白组骨折区未注入任何材料。结果与结论:3组骨折均于术后6周时愈合。X射线片显示,术后2,4,6周,实验组骨折修复区单位面积平均灰度值及成骨细胞计数均明显高于对照组、空白组(P<0.05),且实验组成骨细胞增生活跃。说明可注射温敏携淫羊藿苷壳聚糖水凝胶具有促成骨活性,可促进骨折愈合。

BACKGROUND： Epimedium has in vitro promotive effects on osteoblast osteogenesis, differentiation and maturation Besides, it can stimulate bone formation. OBJECTIVE： To observe the repair effect of injectable thermo-sensitive icariin chitosan hydrogel on radial fracture healing in rabbits. METHODS：The left and right radius was cut off from 36 rabbits under anesthesia and randomly divided into experimental group, control group and blank group. The rabbits in the former two groups were injected with injectable thermo-sensitive icariin chitosan hydrogel and chitosan hydrogel around the fracture ends, respectively. The rabbits in the blank group had no treatment. RESULTS AND CONCLUSION ： Fractures in each group were cured at week 6 after surgery. X-ray films showed that average gray value per unit area and osteoblast count of the experimental group were significantly higher than those of the control and blank groups （P 〈 05）. Moreover, the osteoblast proliferation was active in the experimental group. These results suggest that injectable thermo-sensitive icariin chitosan hydrogel can improve osteoblast activity and promote the fracture healing.