聚磷酸钙纤维/羟基磷灰石/明胶软骨组织工程支架材料的制备及性能

Preparation and properties of calcium polyphosphate fiber/hydroxyapatite/gelatin scaffolds for cartilage tissue engineering

背景:目前明胶基组织工程支架材料存在力学性能低、生物相容性差、降解速率难以控制等缺陷。目的:通过添加聚磷酸钙纤维和羟基磷灰石改善明胶支架材料的性能。方法:以自制聚磷酸钙纤维和羟基磷灰石为添加材料,明胶为基体材料,以戊二醛为交联剂,采用溶媒浇铸/粒子滤取技术制备配比为50/10/40的聚磷酸钙纤维/羟基磷灰石/明胶软骨组织工程支架复合材料。测试支架材料的物理力学性能,并观察其微观结构。结果与结论:采用溶胶凝胶法制得的羟基磷灰石粉末结晶程度较差,经900℃下煅烧0.5h后,可制得结晶程度较高的羟基磷灰石粉末。聚磷酸钙纤维/羟基磷灰石/明胶软骨组织工程支架材料具有三维、连通、微孔网状空间结构,孔隙率在65%-90%之间,满足软骨组织工程对其支架材料孔隙的要求。戊二醛的交联作用和聚磷酸钙纤维的增强作用,克服了明胶在制备多孔支架时容易收缩的缺点,制得高孔隙率三维连通的支架材料。

BACKGROUND： Nowadays, the gelatin scaffold for tissue engineering has many disadvantages, such as low stiffness and strength, bad biocompatibility and uncontrollable degradation rate. OBJECTIVE： To improve the properties of gelatin scaffold by adding calcium polyphosphate fibers and hydroxyapatite METHODS： Calcium polyphosphate fiber and hydroxyapatite were taken as reinforced materials and gelatin was used as matrix materials, besides, glutaric dialdehyde was used as the cross linker in a ratio of 50/10/40. The composite scaffolds for cartilage tissue engineering were prepared by solvent-casting and particulate-leaching technique, and then their physical and mechanical properties as well as microstructure were tested. RESULTS AND CONCLUSION： The hydroxyapatite powder prepared by sol-gel method had a low crystallization degree but it had a high crystallization degree after sintered at 900 ~C for 0.5 hour. The scaffolds with porosity of 65% 90% possessed three-dimensional, interconnecting, micro-hole network structure, and can meet the porosity require of cartilage tissue engineering for scaffolds. The effect of glutaraldehyde cross-linking and improvement of calcium polyphosphate fiber can overcome the disadvantage of easily shrinking of gelatin when the porous scaffold scaffolds are prepared, and provide promise to prepare a high-porosity scaffold material which has three-dimensional and interconnection structure.