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唾液、血清和脓液对电化学测菌法检测牙龈卟啉单胞菌的影响

Influence of saliva,serum and pus on the electrochemical method for Porphyromonas gingivalis detection
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摘要 目的:观察唾液、血清和脓液对电化学测菌法检测牙龈卟啉单胞菌的影响。方法:利用免疫磁分离捕获牙龈卟啉单胞菌后进行电化学阻抗测量,观察唾液、血清和脓液对电化学测菌法检测限和检测灵敏度的影响。结果:唾液对电化学测菌法的检测限无影响,但可降低其检测灵敏度;血清对电化学测菌法的检测限和检测灵敏度均无明显影响;脓液明显影响电化学测菌法对牙龈卟啉单胞菌的检测。结论:应用电化学测菌法检测样本时应避免脓液的污染。 Objective: To investigate the influence of saliva, serum and pus on the electrochemical method for periodontal bacteria detection. Methods: Impedance measurement was carried after immunomagnetic separation of Porphyromonas gingivalis, the influence of saliva, serum and pus on the detection limit and sensitivity of the previously established electrochemical method for the bacteria de- tection was observed. Results: Saliva decreased the detection sensitivity but did not change the detection limit. Serum had no obvious influence on the detection sensitivity and limit of the electrochemical method. Pus could significantly hamper the detection effieeney of the examination method. Conclusion : Pus contamination should be avided for the application of the electrochemical method for bacteria detection.
机构地区 解放军第
出处 《实用口腔医学杂志》 CAS CSCD 北大核心 2012年第6期699-702,共4页 Journal of Practical Stomatology
基金 首都医学发展科研基金(编号:2009-3065) 首都临床特色应用研究基金(编号:D101100050010053)
关键词 电化学测菌法 唾液 血清 脓液 牙龈卟啉单胞菌 Electrochemical method Saliva Serum Pus Porphyromonas gingivalis
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参考文献8

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