摘要
目的探讨磷酯酰肌醇-3-激酶(P13K)、丝氨酸/苏氨酸蛋白激酶(Akt)在糖尿病大鼠骨组织中的表达及意义。方法将50只体重180—200g的6月龄雌性Wistar大鼠按随机数字表法分为对照组(n=24)和2型糖尿病组(n=26)。2型糖尿病组大鼠予高糖高脂饮食喂养8周后,腹腔注射链脲佐菌素30mg/kg,对照组大鼠正常饮食。糖尿病成模后12周两组大鼠分别行腰椎骨密度测定;应用逆转录聚合酶链反应(RT—PCR)法检测骨组织P13K、Aktl和Akt2mRNA的相对表达。组间比较采用t检验。结果对照组大鼠骨密度高于糖尿病组,差异有统计学意义[(0.079±0.034)比(0.060±0.017)g/cm2,t=2.499,P〈0.05];同时,对照组大鼠骨组织P13K、Aktl、Akt2mRNA均高于糖尿病组(分别为:0.65±0.05比0.51±0.04、0.756±O.031比0.694±0.017、0.77±0.04比0.66±0.04),差异均有统计学意义(t=4.969、3.924、4.515,均P〈0.01)。结论2型糖尿病大鼠骨组织P13K、Aktl及Akt2表达低于正常,这可能是2型糖尿病骨质疏松发生的分子生物学机制之一。
Objective To investigate the expression and roles of phosphatidylinositoi-3-kinase (PI3K) , serine/threonine protein kinase(Akt) 1 and Akt2 mRNA in bone of diabetic rats. Methods Fifty female Wistar rats aged 6-month, were randomly divided into control group ( NS group, n = 24) and diabetes group (DM group, n = 26 ). The DM group were made by feeding with high-sugar-fat diet 8 weeks and injected small doses of streptozotocin (STZ) (30 mg/kg), while the NS group were fed with normal diet. After 12 weeks, PI3K, Aktl and Akt2 mRNA in bone were measured respectively by RT-PCR. The t test was used for statistics. Results Compared with the NS group, the bone mineral density (BMD) levels of the DM group was significantly decreased ( ( 0. 079 ± 0. 034 ) vs ( 0. 060 ± 0. 017 ) g,/cm2, t = 2. 499, P 〈 0. 05), and the expression of PI3K, Aktl and Akt2 mRNA in bone tissue of the DM group was also decreased (0. 65 ± 0.05 vs 0. 51 ± 0. 04, 0. 756 ± 0. 031 vs 0. 694 ± 0. 017, 0. 77 ± 0. 04 vs 0. 66 ± 0. 04, t = 4. 969,3. 924 and 4. 515, all P 〈 0. 01 ). Conclusion Decreased expression of PI3K, Aktl and Akt2 mRNA in bone may be one of the pathogenesis in type 2 diabetic osteoporosis.
出处
《中华糖尿病杂志》
CAS
2012年第11期677-680,共4页
CHINESE JOURNAL OF DIABETES MELLITUS
基金
河北省医学科学研究重点课题计划(20120101)
关键词
糖尿病
2型
骨密度
磷酯酰肌醇类
蛋白质丝氨酸苏氨酸激酶
Diabetes mellitus, type 2
Bone mineral density
Phosphatidylinositols
Protein-serine-threonine kinases