新生隐球菌GXM的纯化及其对巨噬细胞甘露糖受体表达调节的研究

Purification of capsular polysaccharide GXM from Cryptococcus neoformans and it's role in regulating MR expression in macrophages

目的从新生隐球菌B3501培养上清中分离和纯化荚膜多糖葡萄糖醛酸木糖甘露聚糖(GXM),观察其是否能调节巨噬细胞甘露糖受体MR的表达。方法采用乙醇沉淀荚膜多糖,十六烷基三甲基溴化铵(CTAB)特异性沉淀方法获得GXM,将GXM与巨噬细胞共孵育24 h,Western blot检测MR的表达变化情况。结果获得了毫克级的GXM,巨噬细胞与GXM孵育后甘露糖受体(mannose receptor,MR)的表达没有明显变化。结论新生隐球菌荚膜多糖GXM不影响巨噬细胞甘露糖受体MR的表达。

Objective To isolate and purificate capsular polysaccharide GXM from Cryptococcus neoformans B3501 and observe whether can GXM regulate MR expression in macrophages. Methods Alcohol was used to precipitate capsular polysaecharide and hexadecyhrimethyl ammonium bromide （CTAB） was used to precipitate GXM specifically. Western blot was used to verify MR ex- pression in macrophages stimulated by GXM for 24 h. Results Up to milligram of GXM was isolated and purificated from the culture supernatant of Cryptococcus neoformans B3501, and MR expression did not change significantly in macrophages stimulated by GXM. Conclusion GXM purificated from Cryptococcus neoformans did not regulate MR expression in macrophages.