Ad-p53与Ad-p16联合对人肺腺癌GLC-82细胞作用的研究

The Experimental Studies of Ad-p53 and Ad-p16 in Combination on Human Lung Adenocarcinoma Cell Line GLC-82

目的：探讨腺病毒介导的ｐ５３与ｐ１６基因联合对人肺腺癌ＧＬＣ－８２疗效提高的可能性。方法：将分别携有ｐ５３基因、ｐ１６基因重组腺病毒（Ａｄ－ｐ５３与 Ａｄ－ｐ１６）联合使用，通过细胞生长和存活实验、克隆形成实验、流式细胞分析、ＴＵＮＥＬ检测、ＲＴ－ＰＣＲ分析、免疫组织化学实验，观察其对人肺腺癌ＧＬＣ－８２细胞的作用。结果：在总感染强度相同的前提下，Ａｄ－ｐ５３与Ａｄ－ｐ１６联合导入ＧＬＣ－８２细胞，对细胞生长存活及克隆形成能力的抑制、以及所造成的凋亡效果比施用单种基因的效果强，表明ｐ５３基因与ｐ１６基因在体外疗效上互为协同。结论：Ａｄ－ｐ５３与Ａｄ－ｐ１６联合，可以提高对人肺腺癌ＧＬＣ－８２细胞的疗效。

Objective: To determine the effect of Ad-53 and Ad-16 both alone and in combination on the growth of human lung adeuocarcinoma cell line GLC-82. Methods: Human lung adenocarcinoma cell line GM-82 were trasferredvia an adenovirus-me- diated p53 gene (Ad-p53), P16 gene Ad-p16 or both.Cell growth inhibition assay, colony formation,flow cytometry assay, TUNEL, RT-PCR and immunhistochemistry assays were used to evaluate the therapeutic efficiency of such strategy on trasferred cells. Results: in vitro experiments, at the same dose of Ad (multiplicity of infection), simultaneous transfer of Ad-p53 and Ad-p16 to GLC-82 cells caused stronger therapeutic efficacy than caused by slope kind of Ad transfer, demonstrating that Ad-p53 treatment has synergistic efficacy with Ad-p16 treatment on GLC-82 cells. cells.Ad-p53 for GLC-82 cells shows enhanced therapeutic efficiency when combined with Ad-p16.