卵巢肿瘤干细胞中趋化因子表达与细胞侵袭后的转移能力

Chemokine expression in the ovarian tumor stem cells and cell invasion and metastasis

背景:利用趋化因子或趋化性多肽招募免疫效应细胞汇集于肿瘤,激发抗肿瘤免疫的方法有望成为肿瘤治疗的新策略。目的:探讨卵巢肿瘤细胞中趋化因子的表达与细胞侵袭转移能力关系。方法:采用RT-PCR方法检测卵巢肿瘤细胞系SW626和Anglne细胞株中CXCR4的表达,然后在Transwell小室检测CXCL12与CXCL12/CXCR4对SW626细胞趋化活性和侵袭活性的影响。结果与结论:RT-PCR检测结果提示SW626卵巢细胞株中有CXCR4分子的表达,而在Anglne细胞中CXCR4无表达。CXCR4的表达能促使卵巢肿瘤细胞中发生趋化与侵袭转移的细胞数目增多,而加入CXCR4中和抗体时,能抑制上述效果。结果可见卵巢肿瘤细胞干细胞趋化因子CXCL12对SW626细胞有明显的趋化活性和侵袭转移活性,其活性是通过其受体CXCR4介导的。

BACKGROUND：The method that using chemokine or chemotactic polypeptide to recruit immune effector cells to converge in tumor cells to arise anti-tumor immunity is expected to become a new strategy for tumor treatment.OBJECTIVE：To discuss the effects of chemokine expression on the invasion and metastasis in the ovarian tumor stem cells for giving new ideas of ovarian cancer research.METHODS：Expression of CXCR4 in ovarian cancer cell line SW626 was detected by reverse transcriptase-polymerase chain reaction（RT-PCR）.Transwell room was used to detect the effects of CXCL12 and CXCL12/CXCR4 on chemotaxic invasion and metastasis of SW626 cells.RESULTS AND CONCLUSION：RT-PCR confirmed that there was CXCR4 expression in the SW626 cell lines,but no expression was found in the Anglne cells.CXCR4 expression could increase the number of ovarian tumor stem cells subjected to chemotactic invasion and metastasis,while CXCR4 addition could inhibit above-mentioned effects.CXCR12,a chemotatic factor of ovarian tumor stem cells,can promote the chemotaxic activity and invasion and metastasis activity mediated by CXCR4 receptor.