不同途径移植骨髓间充质干细胞改善大鼠肝硬化

Different transplantation approaches of bone marrow mesenchymal stem cells in a rat model of cirrhosis

背景:骨髓间充质干细胞移植可减轻肝硬化程度,改善肝功能。目的:观察不同途径移植骨髓间充质干细胞对四氯化碳诱导大鼠肝硬化的作用。方法:将60只SD大鼠随机分为正常组、对照组、门静脉移植组、肝动脉移植组、尾静脉移植组,后4组采用四氯化碳联合乙醇制作肝硬化模型,对照组不进行移植,其余3组分别经门静脉、肝动脉、尾静脉移植大鼠骨髓间充质干细胞1×106。结果与结论:移植4周后,与对照组比较,移植3组大鼠肝功能均得到明显改善,血清白蛋白、胆碱酯酶显著升高(P<0.05),转氨酶、胆红素、凝血时间、Ⅳ型胶原显著降低(P<0.05),肝纤维化程度显著减轻(P<0.05)。门静脉移植组及肝动脉移植组优于尾静脉移植组,前两者之间差异无显著性意义(P>0.05)。说明经门静脉、肝动脉、尾静脉移植骨髓间充质干细胞均可减轻肝纤维化程度,改善肝功能,但肝动脉及门静脉移植途径优于外周血静脉途径。

BACKGROUND：Transplantation of bone marrow mesenchymal stem cells can alleviate cirrhosis and improve liver function.OBJECTIVE：To investigate the differences among the curative effects of three transplantation approaches of mesenchymal stem cells（MSCs） in a rat model of cirrhosis.METHODS：60 SD rats were randomly divided into five groups：normal,control,portal vein transplantation,hepatic artery transplantation and tail vein transplantation groups.The rat model of CC14 and alcohol-induced cirrhosis was prepared in the latter four groups.Rats in the portal vein transplantation,hepatic artery transplantation and tail vein transplantation groups received 1×106 allogenic rat bone marrow mesenchymal stem cells via the portal vein,hepatic artery and tail vein,respectively.The control group rats received no transplantation.RESULTS AND CONCLUSION：Compared with the control group,the liver function of cirrhosis rats in the three transplantation groups was improved significantly,the levels of serum albumin and cholinesterase were significantly increased（P 0.05）,the levels of transaminases,bilirubin,prothrombin time,type IV collagen were significantly decreased,and the degree of cirrhosis was significantly alleviated（P 0.05） at 4 weeks after transplantation in the three transplantation groups.The outcomes were significantly superior in the portal vein transplantation and hepatic artery transplantation groups than in the tail vein transplantation group（P 0.05）.These findings suggest that portal vein transplantation and hepatic artery transplantation can better alleviate the degree of cirrhosis and improve hepatic function than tail vein transplantation,and there is no significant difference between the former two groups.