淫羊藿甙逆转转化生长因子β_2免疫抑制作用的抗肿瘤研究

Reversion of Transforming Growth Factor β_2 Mediated Immunosuppression in Lung Cancer by Icarrin

目的：研究淫羊藿甙（ＩＣＡ）对ＰＧ细胞分泌转化生长因子β２（ｔｒａｎｓｆｏｒｍｉｎｇ ｇｒｏｗｔｈ ｆａｃｔｏｒβ２，ＴＧＦβ２）的影响、ＩＣＡ逆转ＴＧＦβ２介导的免疫抑制作用及其机制。方法：ＭＴＦ法检测细胞增殖、杀伤活性，ＲＴ－ＰＣＲ检测ＴＧＦβ２、穿孔素，流式细胞术检测ＴＧＦβ２、ＩＬ－２Ｒα水平。结果：ＩＣＡ可抑制ＰＧ细胞中ＴＧＦβ２的蛋白和ｍＲＮＡ表达，能够逆转受ＴＧＦβ２抑制的ＬＡＫ和ＣＤ３ＡＫ细胞的杀伤活性，并能部分恢复受ＴＧＦβ２抑制的ＬＡＫ细胞表面ＩＬ－２Ｒα表达和ＣＤ３ＡＫ细胞内穿孔素ｍＲＮＡ水平，以及ＣＤ３ＡＫ细胞的增殖活性。结论：淫羊藿甙通过抑制肿瘤细胞免疫抑制因子ＴＧＦβ２的产生，增强免疫效应细胞的杀伤活性等机制而发挥抗肿瘤作用。

Objective: To investigate the influence of Icarrin(ICA) on transforming growth factor β2(TGFβ2) expression by PG cells and the mechanisms of reversion of TGFβ2 mediated immunosuppression by ICA.Methods: Cell proliferation and cytotoxici- ty were assayed by MTT assay. mRNA level of TGFβ2 and perforin was detected by RT-PCR assay. assay.Expression of TGFβ2 and IL- 2Rα was analysed by folw cytometry assay. Results: ICA could inhibit the protein and mRNA expression of TGFβ2 in PG cells. It could also reverse the supressed cytotoxicity of LAK, CD3AK cells by TGFβ2. The IL-2Rα expression on LAK and perforin gene transcription and proliferation of CD3AK inhibited by TGFβ2 were also partly recovered. Conclusion: The anti-anti-tumor activity of ICA is at least partly due to its capability of inhibiting TGFβ2 expression in tumor cells and restoring the cytotoxicity of immunocompetent cells inhibited by TGFβ2.