大豆异黄酮干预去势大鼠骨密度及成骨细胞雌激素受体α的表达

Effect of soy isoflavones on the bone mineral density and the expression of estrogen receptor alpha in osteoblasts of ovariectomized rats

背景:大豆异黄酮是一类植物雌激素,对绝经后骨质疏松症治疗具有重要意义。目的:进一步验证大豆异黄酮对去势大鼠骨密度和成骨细胞雌激素受体α表达的影响。方法:将12月龄去势雌性SD大鼠随机分为3组,大豆异黄酮组和对照组大鼠摘除双侧卵巢,假手术组只进行手术入路,不切除卵巢。大豆异黄酮组切除卵巢后,每日灌胃大豆异黄酮,连续40d。对照组喂等量生理盐水。结果与结论:喂饲40d后对照组大鼠左侧股骨骨密度值显著低于假手术组(P<0.05),提示骨质疏松模型成功。大豆异黄酮组大鼠左侧股骨骨密度高于对照组(P<0.05);大豆异黄酮组雌激素受体α的表达量高于对照组(P<0.05)。提示大豆异黄酮可增加去势大鼠骨密度,提高去势大鼠成骨细胞雌激素受体α的表达,促进成骨细胞增殖。

BACKGROUND：Soy isoflavones（SIF） is a type of plant estrogen that has significance for osteoporosis after menostasia.OBJECTIVE：To investigate the effect of SIF on the femoral bone mineral density and the expression of osteoblasts estrogen receptor α in osteoblasts of ovariectomized rats.METHODS：Thirty 12-month-old female ovariectomized rats were randomly divided into three groups：sham operation group,SIF group and control group.The rats in the SIF group and control group were excised bilateral ovarian,while the rats in the sham operation group were only performed operative approach without excision of ovarian.After the ovarian was excised,the rats in the SIF group were given SIF every day for 40 days continuously,and the rats in the control group were given the same dose of normal saline.RESULTS AND CONCLUSION：After 40 days,the value of the left femoral bone mineral density in the control group was significantly lower than that of the sham operation group（P0.05）;the expression of estrogen receptor α in osteoblasts of SIF group was higher than that of the control group（P0.05）.These findings suggest that SIF can increase the bone mineral density,improve the expression of estrogen receptor α in osteoblasts of ovariectomized rats,and promote the proliferation of osteoblasts.